The Regulation of Carbon Utilization in Streptomyces

链霉菌碳利用的调控

基本信息

批准号：
9316912
负责人：
Janet Westpheling
金额：
$ 30万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1994
资助国家：
美国
起止时间：
1994-03-01 至 1998-02-27
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9316912&HistoricalAwards=false
关键词：
Regulation Carbon Utilization Streptomyces

项目摘要

9316912 Westpheling Streptomyces are Gram-positive soil bacteria that are of special interest because of their remarkably complex morphological development and because they produce as secondary metabolites most of the antibiotics used in human and animal health care. Although little is understood about the molecular physiology of these bacteria, it is apparent that the regulation of carbon utilization is of central importance in the gene expression pathways for both morphological development and antibiotic biosynthesis. To gain an understanding of the mechanism of catabolite control in Streptomyces we are studying the regulation of a family of genes involved in the utilization of chitin, a complex carbohydrate and a major source of carbon in the soil. The synthesis of chitinase enzymes is tightly repressed by glucose and strongly induced by chitin. In preliminary work we have established that regulation for at least one of the chitin. In preliminary work we have established that regulation for at least one of the chitinase genes, chi63, occurs at the level of transcription initiation. We have been a systematic analysis of mutations within the promoter region of chi63 and used a transcriptional fusion to the xy1E reporter gene, to identify chromosomal mutations that define the pathway for glucose repression and chitin induction of chitinase synthesis. We are defining the elements of the chitinase promoters that determine regulation and promoter activity; beginning a study to identify and characterize genes that act to facilitate the global regulation of catabolite repression in Streptomyces. %%% We hope as a result of this work to contribute to the understanding of gene regulation in an important class of bacteria where relatively little is known about the way in which gene expression is controlled. ***

9316912 Westpheling链霉菌是革兰氏阳性的土壤细菌，由于它们具有非常复杂的形态发展，并且由于它们作为次级代谢产物而产生的大多数用于人类和动物卫生保健中的抗生素。尽管对这些细菌的分子生理学知之甚少，但很明显，碳利用的调节对于形态学发育和抗生素生物合成的基因表达途径至关重要。为了了解链霉菌中分解代谢物控制的机制，我们正在研究涉及甲壳质利用，复杂碳水化合物的基因家族，土壤中碳的主要来源。几丁质酶的合成被葡萄糖严重抑制，并由几丁质诱导。在初步工作中，我们确定了至少一种几丁质的调节。在初步工作中，我们确定至少有几丁质酶基因Chi63的调节发生在转录启动水平。我们一直是对Chi63启动子区域内突变的系统分析，并将转录融合到XY1E报告基因中，以鉴定染色体突变，这些突变定义了葡萄糖抑制途径和几丁质酶合成的途径。我们正在定义决定调节和启动子活性的几丁质酶启动子的元素；开始一项研究，以识别和表征起作用的基因，以促进链霉菌中分解代谢物抑制的全球调节。 %%％我们希望这项工作有助于在重要类细菌中对基因调节的理解，在这种细菌中，对基因表达的控制方式相对较少。 ***