Regulation of secondary metabolism and cell differentiation by A-factor and protein phosphorylation in Streptomyces.

链霉菌中 A 因子和蛋白质磷酸化对次生代谢和细胞分化的调节。

• 批准号: 06454073
• 负责人: HORINOUCHI Sueharu
• 金额: $ 4.86万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454073/
• 关键词: Streptomycetes; Utoregulator; Antibiotic; Morphogenesis; Signal transduction; A-ファクター; 抗生物質生産

(1) The A-factor recepor protein (ArpA) was purified from S.griseus and arpA was cloned by PCR.We also cloned two arpA homologs from S.coelicolor A3 (2), which probably controlled antibiotic production in this strain, as determined by the phenotypes of each of disruptants.(2) Transcription of amfR,a regulatory gene for aerial mycelium formation in S.griseus, was controlled by A-factor. A protein able to bind to its promoter region was purified and its gene was cloned.(3) An afsK homolog (afsK2) was cloned from S.griseus. Genetic studies showed that the afsK2 gene is involved in aerial mycelium formation under the conditons of high osmolality(4) Upstream from afsA was present a gene encoding a protein similar to prokaryotic transcriptional factors. The expression of afsA therefore apeared to be controlled by this upstream gene.(5) An A-factor-responsive transcriptional activator (Adp) that bindsto the strR promoter was purified to homogeneity. The molecular mass of Adp was 40 kDa.(6) A tyrosine phosphatase gene was cloned from S.coelicolor A3 (2) as a gene that caused pigmentation in S.lividans. The phosphatase produced in E.coli showed a tyrosine phosphatase activity.

（1）从s.griseus和ARPA纯化A因子回收蛋白（ARPA）。我们还通过s.coelicolor A3（2）克隆了两个ARPA同源物，这些ARPA同源物可能由该菌株中控制的抗生素产生，如DINSPERCRIANS所确定的（2）eNculter的频率。 S.Griseus由A因子控制。纯化了能够与其启动子区域结合的蛋白质，并克隆其基因。（3）AFSK同源物（AFSK2）从S.Griseus克隆。遗传研究表明，在高渗透压（4）上游的高渗透压（4）下，AFSK2基因参与了空中菌丝体的形成（4）是一种基因，该基因编码类似于原核生物转录因子的蛋白质。因此，AFSA的表达被该上游基因控制。（5）A因子响应转录激活剂（ADP）将bindsto bindsto strr启动子纯化为同质性。 ADP的分子质量为40 kDa。（6）酪氨酸磷酸酶基因被从S.coelicolor A3（2）克隆为导致S.lividans色素沉着的基因。大肠杆菌中产生的磷酸酶显示出酪氨酸磷酸酶活性。

项目成果

Kudo, N., M.Kimura, T.Beppu, and S.Horinouchi: "Cloning and characterization of a gene involved in aerial mycelium formation in Streptomyces griseus" J.Bacteriol. 177. 6401-6410 (1995)

Kudo, N.、M.Kimura、T.Beppu 和 S.Horinouchi：“灰色链霉菌气生菌丝体形成相关基因的克隆和表征”J.Bacteriol。

S.Horinouchi: "Dynamic Aspect of Natural Products chemistry-Molecular Biological Approaches" Kodansha-Scientific(印刷中),

S. Horinouchi：“天然产物化学的动态方面 - 分子生物学方法”讲谈社科学（出版中），

Horinouchi, S., and T.Beppu: "Autoregulators. Genetics and Biochemistry of Antibiotic Production. Butterworth-Heinemann Publishers, Boston" 103-119 (1994)

Horinouchi, S. 和 T.Beppu：“自动调节器。抗生素生产的遗传学和生物化学。Butterworth-Heinemann 出版社，波士顿”103-119 (1994)

Sueharu Horinouchi: "Signal transduction via A-factor that controls antibiotic production and morphogenesis in Streptomyces." Proceedings of International Symposium on Functional Biomaterials. 88-98 (1995)

Sueharu Horinouchi：“通过 A 因子进行信号转导，控制链霉菌中抗生素的产生和形态发生。”

A.Matsumoto: "Phosphorylation of the AfsR protein involved in secondary metabolism in Streptomyces spp. by a eukaryotic-type proteinkinase" Gene. 146. 47-56 (1994)

A.Matsumoto：“真核型蛋白激酶对参与链霉菌属次生代谢的 AfsR 蛋白进行磷酸化”基因。