Japan-UK Joint Study on Molecular Genetics of Streptomyces

日英链霉菌分子遗传学联合研究

• 批准号: 09044205
• 负责人: HORINOUCHI Sueharu
• 金额: $ 4.61万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044205/
• 关键词: Japan-UK Joint Study; Streptomyces; Molecular Biology; Antibiotics Production; Genetic Engineering; Molecular Breeding; Secondary Metabolism; Morphological Differentiation; Streptomyces

The purpose of this study is promoting Japan-UK joint researches on molecular genetics of Streptomyces. A workshop on molecular genetics of Streptomyces was held at John Innes research center (Norwich, UK) on October 1997. Fifteen Japanese researchers took part in the workshop and discussed about joint researches. The proceeding of the workshop was distributed to many researchers who study on Streptomyces.In the second year, 1998, three professors of John Innes research center (Sir. D.Hopwood, K.F.Chater and M.J.Bibb) visited Japan and discussed about joint researches with Japanese researchers. S.Horinouchi, head investigator of this study, visited John Innes research center on September. Y.Ohnishi, one of inverstigators of this study, stayed for three weeks at John Innes research center. He made experiments with some researchers of the center.Thanks to this study, many joint researches started and more intimate relationship was established between Japanese and English researchers.

这项研究的目的是促进对链霉菌分子遗传学的日本-UK联合研究。 1997年10月，在约翰·英恩斯研究中心（英国诺里奇）举行了有关链霉菌分子遗传学的研讨会。15名日本研究人员参加了研讨会，并讨论了有关联合研究的讨论。研讨会的程序分发给了许多研究链霉菌的研究人员。在1998年第二年，约翰·英恩斯研究中心（John Innes Research Center）的三位教授（D.Hopwood，K.F.Chater和M.J.Bibb）访问了日本，并与日本研究人员讨论了联合研究。这项研究的首席调查员S. Horinouchi于9月访问了John Innes Research Center。 Y.Ohnishi是这项研究的无名者之一，在John Innes Research Center住了三个星期。他与中心的一些研究人员进行了实验。感谢这项研究，许多联合研究开始了，日本和英国研究人员之间建立了更亲密的关系。

项目成果

Kawamoto, S.: "Expression analysis of the ssgA gene product, associated with sporulation and cell division in Streptomyces griscus" Microbiol.143. 1077-1086 (1997)

Kawamoto, S.：“与灰色链霉菌孢子形成和细胞分裂相关的 ssgA 基因产物的表达分析”Microbiol.143。

Labes, G.: "Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage 119 using the gentamicin resistance gene (aacCl) of Tn 1696 as reporter" Microbiology. 143. 1503-1512 (1997)

Labes, G.：“使用 Tn 1696 的庆大霉素抗性基因 (aacCl) 作为报告基因，从加纳链霉菌噬菌体 119 中分离和表征强启动子元件”微生物学。

Chakrabutryy, R.: "The ppGpp synthetase gene (rclA) of Streptomyces coelicolor A3(2) plays a conditional role in antibiotic production and morphological differentiation" J. Bacteriol.179. 5854-5861 (1997)

Chakrabutryy, R.：“天蓝色链霉菌 A3(2) 的 ppGpp 合成酶基因 (rclA) 在抗生素生产和形态分化中发挥着条件作用”J. Bacteriol.179。

Kenji Ueda: "Stimulatory effect of copper on antibiotic production and morphological differetiution in Strepromyses tonshipnsis" J. Antibiot.50. 693-695 (1997)

Kenji Ueda：“铜对 Strepromyses tonshipnsis 抗生素产生和形态分化的刺激作用”J. Antibiot.50。

Miyoko Waki: "cloning and Characterization of the gene (favA) encoding the receptor for an extracellular regulatory fuctor (2M-2) form strepromyces sp strain PRL-Y" J. Bacteriol.179・16. 5131-5137 (1997)

Miyoko Waki：“编码链霉菌 PRL-Y 菌株细胞外调节子 (2M-2) 受体的基因 (favA) 的克隆和表征” J. Bacteriol.179·16 (1997)。