Taxol Biosynthesis: Identity of Taxol Direct Precursor in Cell Culture

紫杉醇生物合成：细胞培养中紫杉醇直接前体的身份

• 批准号: 9632961
• 负责人: Michael Shuler
• 金额: $ 5万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-15 至 1998-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9632961&HistoricalAwards=false
• 关键词: Taxol; Biosynthesis; Identity; Direct; Precursor

9632961 Shuler The goal of this proposed research is to identify and investigate the biosynthetic significance of an unknown taxane recently discovered in the Principle Investigators laboratory while performing plant tissue culture experiments with certain metabolic inhibitors. These experiments involved the culture of Taxus species which produce Taxol, an important new anti-cancer drug. It is currently generally accepted that baccatin III is the direct biosynthetic precursor of Taxol. However, recent experiments by the PI indicate that baccatin III could be selectively and completely inhibited without any significant effect on Taxol production. Other inhibition experiments have shown the accumulation of a single unknown taxane, which is not baccatin III, but which could,, in fact, be the direct precursor of Taxol. This proposal outlines a multidisciplinary approach to identify this unknown compound and investigate its biosynthetic significance using knowledge of manipulation of Taxol production by Taxus cell cultures, and established techniques in biosynthetic chemistry. ***

9632961 Shuler这项拟议的研究的目标是识别和研究主要研究人员实验室中最近发现的未知紫杉烷的生物合成意义，同时对某些代谢抑制剂进行了植物组织培养实验。 这些实验涉及产生紫杉醇的出租车物种的培养，这些物种是一种重要的新抗癌药。 目前普遍认为Baccatin III是紫杉醇的直接生物合成前体。 但是，PI的最新实验表明，巴卡蛋白III可以被选择性地抑制，而不会对紫杉醇产生产生任何重大影响。 其他抑制实验表明，单个未知紫杉烷的积累，这不是巴卡蛋白III，但实际上可能是紫杉醇的直接前体。 该提案概述了一种多学科方法来识别这种未知化合物，并使用对紫杉醇细胞培养物对紫杉醇生产的操纵以及在生物合成化学中建立的技术进行了研究。 ***