Overcoming Barriers to Protein Production in Plant Cell Cultures

克服植物细胞培养中蛋白质生产的障碍

• 批准号: 0109936
• 负责人: Michael Shuler
• 金额: --
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0109936&HistoricalAwards=false
• 关键词: Overcoming; Barriers; Protein; Production; Plant

The objective of this project is to identify bottlenecks in heterologous protein production from transgenic plant cell suspensions, and to develop genetic strategies to overcome these limitations. Three model proteins will be studied. These include, hepatitis B surface antigen, Norwalk virus capsid protein, and human secreted alkaline phosphatase. These proteins will be studied in two host systems, i. e. tobacco and soybean suspension cultures. The Principal Investigators (PIs) will test the hypothesis that transcribed mRNA may have increased opportunities for translation in the late exponential/stationary phase as compared with the exponential growth phase due to competition with endogenous mRNA for translational machinery. If this is true, then inducible expression during the late exponential growth/stationary phase might lead to higher product levels.

该项目的目的是从转基因植物细胞悬浮液中鉴定出异源蛋白质产生的瓶颈，并制定遗传策略以克服这些局限性。 将研究三个模型蛋白。 其中包括丙型肝炎表面抗原，Norwalk病毒衣壳蛋白和人分泌的碱性磷酸酶。 这些蛋白质将在两个宿主系统中进行研究。 e。烟草和大豆悬浮培养物。 主要研究者（PIS）将检验以下假设：由于与内源性mRNA竞争转化机械的竞争，与指数增长阶段相比，转录的mRNA可能在指数晚期/固定阶段增加了翻译的机会。 如果这是真的，那么在指数晚期的生长/静止期间的诱导表达可能会导致较高的产品水平。