Acquisition of Biophysics Instrumentation for Core Facility

为核心设施购置生物物理仪器

• 批准号: 9512577
• 负责人: Ronald Raines
• 金额: $ 44.24万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9512577&HistoricalAwards=false
• 关键词: Acquisition; Biophysics; Instrumentation; Core; Facility

Funds are requested for 50% of the cost of creating the University of Wisconsin-Madison Facility for Biophysics Instrumentation. This campus facility would be part of a $30 million expansion of the Department of Biochemistry. The facility would house six major instruments for the study of the complexation, conformation, and structure of biological and other macromolecules. These instruments are a MicroCal isothermal titration calorimeter, Calorimetry Sciences differential scanning calorimeter, Beckman analytical ultracentrifuge, Aviv circular dichroism spectrometer, Nicolet Fourier transform infrared spectrometer, and Pharmacia surface plasmon resonance detector. In addition, an Instrumentation Specialist would be hired to provide technical assistance to students, staff, and faculty on the installation, use, and maintenance of the requested instruments. The six principal users of the requested instrumentation are faculty members who use biophysical methods to study biopolymers and their interactions. These faculty reside in four divisions of the university- -Biochemistry, Chemistry, Chemical Engineering, and Pharmacy. They have active collaborations, routinely share equipment and ideas, and participate in common training and degree programs. Together, they would manage the operations of the proposed core facility. The major users of the requested instruments would be the approximately 100 students and staff being trained in the laboratories of the primary users. In addition, the requested instruments would greatly enhance the research and training efforts of ten secondary users. The laboratories of all primary and secondary users are within two city blocks of the proposed facility. Further, it is envisioned that the usage and hence the impact of the facility would soon broaden well beyond the research groups of the primary and secondary users. The need for the requested instrumentation is extreme. Physical approaches to the analysis of biological systems depend heavily on instrumentation. Researchers at the University of Wisconsin-Madison now have the ability to produce biomolecules on a large scale, and to perform limited physical analyses on these molecules. Yet, no isothermal titration calorimeters, no differential scanning calorimeters, no analytical ultracentrifuges, and no surface plasmon resonance detectors exist on any campus of the University of Wisconsin. The Madison campus has two antiquated circular dichroism spectrometers and only three Fourier transform infrared spectrometers, each of which is inaccessible to the primary and secondary users. This proposal is intended to address this crippling lack of modern biophysical instrumentation.

要求资金用于创建威斯康星大学麦迪逊分校的生物物理学仪器的50％。该校园设施将成为生物化学系扩建3000万美元的一部分。该设施将容纳六种主要工具，以研究生物和其他大分子的络合，构象和结构。 这些仪器是微局部等温滴定量热计，量热法差异扫描量热量计，贝克曼分析超级中学分析，AVIV圆形二分法光谱仪，Nicolet傅立叶傅立叶变换红外光谱仪和Pharmacia Surface Plasmon Surface Plasmon Consonance vistector。此外，将聘请仪器专家为学生，教职员工和教职员工提供所需工具的安装，使用和维护。 所请求的仪器的六个主要用户是使用生物物理方法研究生物聚合物及其相互作用的教师。这些教师居住在大学的四个部门 - 化学，化学工程和药房。他们有积极的合作，经常共享设备和想法，并参加了共同的培训和学位课程。他们将共同管理拟议的核心设施的运营。所请求的乐器的主要用户将是在主要用户实验室接受培训的大约100名学生和员工。此外，所要求的工具将大大改善十个二级用户的研究和培训工作。所有初级和二级用户的实验室都在拟议设施的两个城市街区内。此外，可以预见的是，该设施的用法及其影响很快将范围扩大到主要和二级用户的研究小组之外。 对请求的仪器的需求是极端的。生物系统分析的物理方法在很大程度上取决于仪器。威斯康星大学麦迪逊分校的研究人员现在有能力大规模生产生物分子，并对这些分子进行有限的物理分析。但是，威斯康星大学的任何校园内都没有等温滴定热量表，没有差分扫描量热量表，没有分析性超速离心物，并且没有表面等离子体共振探测器。 麦迪逊校园有两个过时的圆形二分色谱仪，只有三个傅立叶变换红外光谱仪，每个红外光谱仪对主要和次要用户都无法访问。该建议旨在解决这种严重的现代生物物理仪器。