Engineering Research Equipment: Total Internal Reflectance Fluorescence (TIRF) for Bioengineering at Interfaces

工程研究设备：用于界面生物工程的全内反射荧光 (TIRF)

• 批准号: 9500468
• 负责人: Kathleen Stebe
• 金额: $ 7万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9500468&HistoricalAwards=false
• 关键词: Engineering; Research; Equipment; Total; Internal

9500468 Stebe The equipment grant provides funds to purchase equipment to construct a photon-counting total internal reflectance fluorescence (TIRF) apparatus and a TIRF microscope with video- digitization and photobleaching fluorescence recovery capabilities to establish the Laboratory for Bioengineering at Interfaces (LBI). TIRF methods are non-invasive, non- destructive, and well suited for kinetic studies, as real time data can be gathered on time scales ranging from seconds to hours. The fluorescence methods will be used to study dynamic events at interfaces in three research area: 1) interactions of proteins and block copolymers with engineered bilayers, 2) cellular adhesion and aggregation to biomimetic surfaces, and 3) protein adsorption at hydrogel-polymer interfaces in synthetic membranes. The work carried out as described is expected to produce fundamental knowledge in the field of molecular and cell dynamics at interfaces, and lead to new discoveries. ***

9500468设备拨款提供了购买设备的资金，以构建光子计数总内反射荧光荧光（TIRF）设备（TIRF）设备和带有视频数字化和光泽荧光回收能力的TIRF显微镜，以在接口（LBI）（LBI）建立实验室的实验室。 TIRF方法是无创，无破坏性的，并且非常适合动力学研究，因为可以按时间尺度收集从几秒钟到几个小时的时间尺度收集实时数据。 荧光方法将用于研究三个研究领域的界面的动态事件：1）蛋白质和与工程双层的共聚物的相互作用，2）2）细胞粘附和对生物模拟表面的聚集，以及3）蛋白质吸附在合成膜中的水凝胶聚合物接口处的蛋白质吸附。 如上所述，进行的工作将在界面的分子和细胞动力学领域产生基本知识，并导致新发现。 ***