Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia

产溶剂梭菌的遗传分析和代谢工程

• 批准号: 9210108
• 负责人: Eleftherios Papoutsakis
• 金额: $ 68.79万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-15 至 1999-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9210108&HistoricalAwards=false
• 关键词: Genetic; Analysis; Metabolic; Engineering; Solventogenic

The objectives of this project are to produce new knowledge on the molecular genetics and enzymology of anaerobic butyric clostridia that will allow beneficial genetic alterations of the cell metabolism for a variety of applications. Butyric acid clostridia have an excellent potential for industrial applications to produce commodity and specialty chemicals. The current knowledge base on the genetics, regulation and metabolism of these cells has been limited, but considerable progress has been made by work on previous grants. This project will continue the study of gene regulation, develop further the expression systems in clostridia, and evaluate other factors involved in the regulation of metabolic pathways in clostridia.

该项目的目标是产生关于厌氧丁酸梭菌的分子遗传学和酶学的新知识，这将使细胞代谢的有益遗传改变用于各种应用。 丁酸梭菌在生产商品和特种化学品的工业应用中具有巨大的潜力。 目前关于这些细胞的遗传学、调控和代谢的知识基础有限，但之前的资助工作已经取得了相当大的进展。 该项目将继续研究基因调控，进一步开发梭菌表达系统，并评估参与梭菌代谢途径调控的其他因素。