The Use of Immunocytochemical Techniques for Phytoplankton Growth Rate Estimation Via Cell Cycle Analysis

使用免疫细胞化学技术通过细胞周期分析估计浮游植物生长速率

• 批准号: 9115618
• 负责人: Gordon Taylor
• 金额: $ 27.88万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9115618&HistoricalAwards=false
• 关键词: Use; Immunocytochemical; Techniques; Phytoplankton; Growth

Single-cell techniques for measuring species-specific growth rates and quantum yield in situ have been developed and tested. The growth rates of individual species of marine phytoplankton can be determined from data on a species' diel cycle of DNA replication. The species-specific quantum yield is determined from single-cell absorption spectra and the spectral distribution of daily integrated light intensity. These techniques have been tested in field conditions, and the results agree with related lab studies. In this research, Dr. Carpenter and collaborators will develop a simpler and more widely applicable method of measuring growth rates by using cell cycle markers. This new approach will be less time consuming, eliminate the requirement for sophisticated equipment, and allow the production of permanent slides for record keeping. A technique for separating phytoplankton cells from zooplankton and detrital particles will be developed to make these measurements possible. In addition, these techniques will be applied to study the causes of phytoplankton succession in Long Island Sound and to test the hypothesis that the change from a diatom dominated community to a dinoflagellate dominated one is caused by the alteration of intrinsic growth rates in corresponding populations.

已经开发和测试了用于测量物种特异性生长速率和原位量子产率的单细胞技术。 海洋浮游植物的个别物种的生长速率可以从物种DNA复制周期的数据中确定。 物种特异性的量子产率是由单细胞吸收光谱和每日综合光强度的光谱分布确定的。 这些技术已在现场条件下进行了测试，结果与相关的实验室研究一致。 在这项研究中，Carpenter博士和合作者将通过使用细胞周期标记来开发一种更简单，更广泛地适用于测量增长率的方法。 这种新方法将减少耗时，消除对复杂设备的需求，并允许生产永久幻灯片以保持记录。 将开发出一种将浮游植物细胞与浮游物颗粒分离的技术，以使这些测量成为可能。 此外，这些技术将应用于研究长岛声音中浮游植物延长的原因，并检验以下假设：从硅藻主导的群落变为鞭毛鞭毛的变化是由相应种群中内在生长速率的改变引起的。