Collaborative Research: Microbial Observatory in the Cariaco Basin - Dynamics of Protistan Diversity across Time, Space, and Chemical Gradients

合作研究：卡里亚科盆地微生物观测站 - 跨时间、空间和化学梯度的原生生物多样性动态

• 批准号: 0348442
• 负责人: Gordon Taylor
• 金额: --
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0348442&HistoricalAwards=false
• 关键词: Collaborative; Research; Microbial; Observatory; Cariaco

The long term goal of this project is to build a biosphere-wide record of protistan life in time and space and elucidate their roles in global ecology and biogeochemistry. The project is obtaining an exhaustive record of protistan diversity and its dynamics in the largest marine anoxic system, the Cariaco Basin. The project is: conducting a comprehensive survey of protistan 18S rDNA diversity in suboxic and anoxic habitats in the Cariaco Basin 1) by sampling several ecological guilds in the water column and sediments, by systematic recovery of the protistan 18S rDNA sequences, by extensive coverage of the clone libraries and by analysis of the phylogenetic positions of the detected protists; 2) by recovering numerically and phylogenetically significant species for direct SEM examination and characterization of their properties by using new methodology including FISH and light and SEM ultrastructural characterization; efforts are being made to enrich for and cultivate unique species for physiological, biochemical and morphological investigations; 3) by assessing the ecological importance of recovered protistan species by using FISH on representative sets of environmental samples and quantitatively describing the species dynamics over time and space and across environmental gradients and 4) by disseminating information resulting from the project via a Knowledge Center of Protistan Diversity by integrating the traditional taxonomy with the molecular data and compiling the information on species identification, occurrence and distribution. The internet-based Knowledge Center is working in concert with other data sets, principally micro*scope (http://www.mbl.edu/microscope), created by one of the co-PIs. Broader impacts of the project include the integration of data into modern knowledge network technologies and training a large number of graduate students and undergraduates, including students of Venezuelan collaborators. This project is working closely with the Chistoserdov project in the Cariaco Basin, where the focus is on the investigation of prokaryotic diversity in the redox zone of the basin.

该项目的长期目标是在时空建立范围内的生物圈记录，并阐明其在全球生态学和生物地球化学中的作用。 该项目正在获得最大的海洋缺氧系统Cariaco盆地的详尽记录及其动态。 该项目是：通过在水柱和沉积物中在cariaco盆地中对Pitistan 18S rDNA多样性进行全面调查，通过对Protistan 18S RDNA序列进行系统的恢复，通过对锁骨图书馆进行广泛的覆盖范围，并通过分析蛋白质的覆盖，通过对水柱的系统恢复进行系统的恢复，并通过分析蛋白质的覆盖来进行; 2）通过使用新方法，包括鱼类和光和SEM超微结构表征，通过数值和系统发育有意义的物种进行直接进行SEM检查和特性的表征；正在努力为生理，生化和形态学研究而丰富和培养独特的物种； 3）通过在环境样本的代表性集中使用鱼类来评估恢复的原生物物种的生态重要性，并定量描述随时间和空间以及整个环境梯度的物种动态，以及4）4）通过将项目的知识中心传播到项目的知识中心，通过将传统分类与分子数据进行整合并构成物种认同的信息，并分配了物种的信息和分配。 基于Internet的知识中心正在与其他数据集（主要*范围（http://www.mbl.edu/microscope）共同使用，该数据集由其中一个共同PIS创建。该项目的更广泛影响包括将数据集成到现代知识网络技术中，并培训大量的研究生和本科生，包括委内瑞拉合作者的学生。 该项目正在与Cariaco盆地的Chistoserdov项目紧密合作，该项目的重点放在研究盆地氧化还原区的原核多样性的研究上。