The use of Immunocytochemical Techniques for Phytoplankton Growth Rate Estimation Via Cell Cycle Analysis

使用免疫细胞化学技术通过细胞周期分析估计浮游植物生长速率

• 批准号: 9529970
• 负责人: Edward Carpenter
• 金额: $ 31万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9529970&HistoricalAwards=false
• 关键词: use; Immunocytochemical; Techniques; Phytoplankton; Growth

9529970 Taylor The primary goal of this research is to advance and use a method, which can easily by employed by the scientific community, for measuring species specific growth rates of phytoplankton. The method follows a progression of developments from the frequency of dividing cells technique, to the diel DNA synthesis method, to the cell cycle approach for measuring growth rates. With this method a conserved cell cycle protein, PCNA (proliferating cell nuclear antigen) is used as a marker of cells which are about to divide. Fluorescent antibodies are used to detect PCNA, and a standard epifluorescence microscope is used for detecting presence or absence of antibodies and identifying species of phytoplankton, thus making the method available to a broad range of investigators. This research builds upon and advances established methodology and will benefit a large segment of the biological oceanographic community. One of the major goals of plankton biologists is to understand the causes of blooms and the contribution of individual species as primary producers. The research will establish a sound, fast affordable and rugged method for measuring species specific growth rates, and will also provide basic information on cell cycle proteins in phytoplankton. ***

9529970泰勒这项研究的主要目标是进步和使用科学界使用的方法，可以轻松地测量浮游植物的特定物种生长速度。 该方法遵循从分裂细胞技术到DIEL DNA合成方法到测量生长速率的细胞周期方法的发展发展。 使用这种方法，保守的细胞周期蛋白，PCNA（增殖细胞核抗原）用作即将分裂的细胞标记。 荧光抗体用于检测PCNA，并使用标准的落荧光显微镜来检测存在或不存在抗体并识别浮游植物的物种，从而使该方法可用于广泛的研究者。 这项研究以建立方法论为基础并进步，并将使生物海洋学界的大部分地区受益。 浮游生物生物学家的主要目标之一是了解开花的原因以及单个物种作为主要生产者的贡献。 这项研究将建立一种良好的，快速的负担得起和坚固的方法，用于测量物种特定的生长速率，还将提供有关浮游植物中细胞周期蛋白的基本信息。 ***