Molecular Genetic Analysis of det loci of Arabiodopsis thaliana

拟南芥基因座的分子遗传学分析

基本信息

批准号：
8904106
负责人：
Joanne Chory
金额：
$ 33.68万
依托单位：
The Salk Institute for Biological Studies
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1989
资助国家：
美国
起止时间：
1989-09-01 至 1993-02-28
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=8904106&HistoricalAwards=false
关键词：
Molecular Genetic Analysis det loci

项目摘要

The overall objective of this project is to elucidate the pathway of sensory perception associated with chloroplast development in plants. The process of greening, or differentiation of the chloroplast, involves the coordinate regulation of many nuclear and chloroplast genes. The cues for the initiation of this developmental program are both extrinsic (light) and intrinsic (cell-type and plastid signals). Several regulatory photoreceptor are involved in the perception of light signals, but nothing is known of the transduction elements that mediate either the external or intracellular signals. The proposed research program focusses on a novel class of Arabidopsis thaliana mutants previously isolated by the principal investigator. The mutant plants develop light-grown characteristics even when grown in darkness, including, leaf development and anthocyanin accumulation and are therefore designated det, for de-etionlated. There are at least 2 det complimentation groups. The specific strategic approach will be to characterize genetically, biochemically, and physiologically the previously identified det mutant, as well identify new det mutant classes. Double mutants will be made to begin analysis of gene interaction, which will eventually lead to construction of the path(s) which lead to greening. The characterization of these mutants has already suggested that the response to light may be a repression rather than an induction phenomenon. Further work promises to elucidate the greening pathways in plants, pathway which must be understood for practical as well as basic reason.

该项目的总体目的是阐明与植物中叶绿体发育相关的感觉知觉的途径。绿色或叶绿体分化的过程涉及许多核和叶绿体基因的坐标调节。启动此发展程序的提示既是外在（光）和内在（细胞类型和质体信号）。几种调节感受器参与了光信号的感知，但是对介导外部或细胞内信号的转导元件一无所知。拟议的研究计划的重点是先前由首席研究者隔离的新型拟南芥突变体。即使在黑暗中生长，包括叶片发育和花青素的积累，也会形成突变植物，即使在黑暗中生长，也被指定为DET。至少有2个DET互补组。具体的战略方法是从遗传，生化和生理上表征先前鉴定的DET突变体，并确定新的DET突变类别。将使双突变体开始分析基因相互作用，这最终将导致导致绿化的路径的构造。这些突变体的表征已经表明，对光的反应可能是一种抑制，而不是诱导现象。进一步的工作有望阐明植物中的绿色途径，这是由于实际和基本原因必须理解的途径。