Brassinosteroid-auxin synergism to dissect hormone response pathways in Arabidopsis

油菜素类固醇-生长素协同作用剖析拟南芥中的激素反应途径

• 批准号: 0649389
• 负责人: Joanne Chory
• 金额: $ 72万
• 依托单位: The Salk Institute for Biological Studies
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0649389&HistoricalAwards=false
• 关键词: Brassinosteroid; auxin; synergism; dissect; hormone

A major unanswered question in developmental biology is how the final size of an animal or plant is determined. This study explores the roles of hormones in regulating organ size and stature in plants. Recent studies in the P.I.'s laboratory indicate that the plant hormones auxin and brassinosteroids (BRs) act in close proximity to regulate seedling growth. Using genetics, genomics, and physiological analyses, it was shown that the auxin and BR pathways are interconnected and this integration is likely to occur at the level of gene expression. This project will continue to explore the underlying molecular mechanism of this BR/auxin synergism and seek to identify new factors involved in growth regulation by BRs and auxin. These experiments will address the mechanism by which these hormones interact to regulate such diverse responses as changes in gene expression, cell expansion and division, and tissue differentiation. The long-term objective is to completely understand the signaling networks that regulate plant development in response to an ever-changing environment, and to relate this to strategies used in other organisms. An anticipated broader impact is a continuing commitment to post-graduate, graduate, and undergraduate education, as well as exposure of high school students to a research laboratory setting. Also, the computational analyses to be carried out allow access to publicly available datasets that are of general use to the molecular biology community, and we have made the information available through several recently implemented websites. Consequently, the analytical methods and the information generated by their implementation may be a valuable community resource.

发育生物学中的一个主要问题是如何确定动物或植物的最终大小。这项研究探讨了激素在调节植物中器官大小和身材的作用。 P.I.实验室的最新研究表明，植物激素生长素和腕足激素（BRS）在调节幼苗生长方面的近距离起作用。 使用遗传学，基因组学和生理分析，表明生长素和BR途径是相互联系的，并且这种整合可能在基因表达水平上发生。该项目将继续探索这种BR/生长素协同作用的基本分子机制，并试图确定BRS和生长素与生长调节有关的新因素。这些实验将解决这些激素相互作用以调节基因表达，细胞膨胀和分裂以及组织分化等多种反应的机制。长期目标是完全理解根据不断变化的环境来调节植物开发的信号网络，并将其与其他生物中使用的策略联系起来。预期的更广泛的影响是对研究生，研究生和本科教育的持续承诺，以及高中生接触研究实验室环境。此外，要进行的计算分析允许访问分子生物学社区一般使用的公开数据集，并且我们通过最近实施的网站提供了信息。因此，分析方法及其实施产生的信息可能是宝贵的社区资源。