DNA甲基化调控核糖体基因转录延长的功能机制研究

Epigenetic modifications interchange beteewn active and silent modifications, and through which the dynamics of epigenetic modifications regulate gene transcription. With in-depth research, epigenetic modifications have been given new regulatory functions beyond old ones. DNA methylation, an important epigenetic modification, has been shown to exist with a dynamic methylation / demethylation process in the promoter, and may be enriched in coding regions to engage in the regulation of transcription elongation. This application project seeks to study the regulation mechanism of DNA methylation on ribosomal gene tanscription, in particular to explore the molecular relationship of DNA methylation and transcription elongation, to analyze how DNA methylation (5-mC and 5-hmC methylation) is involved in transcription elongation, and explore the coordination between DNA methylation and silent histone modifications during transcription elongation. This study will provide new information for deep research on the functions of DNA methylation to conrol gene transcription.

表观遗传修饰能以转录活跃修饰和转录抑制修饰之间相互转换的动态变化方式，来调控基因转录的过程。随着研究的深入，表观遗传的各种修饰方式不断地被赋予新的调控功能。DNA甲基化，一个重要的表观遗传修饰，被证明在启动子区域存在动态的甲基化/去甲基化过程，并可能富集在编码区参与转录延长的调控。本申请项目试图研究核糖体基因转录中DNA甲基化调控基因转录的分子机制，尤其是探究DNA甲基化与核糖体基因转录延长的功能作用关系，分析DNA甲基化（5-mC甲基化及 5-hmC甲基化）参与转录延长的机制，探索DNA甲基化伴随的抑制性组蛋白修饰与转录延长的协调关系，研究核糖体基因启动子区转录起始中可能的DNA甲基化/去甲基化循环的过程和与转录起始的功能关系，为深入了解DNA甲基化对基因转录的控制机制提供新的研究信息。