Next generation of microfluidic nanosensing platforms for medical diagnostics and environmental monitoring

用于医疗诊断和环境监测的下一代微流控纳米传感平台

• 批准号: RGPIN-2020-04580
• 负责人: SanatiNezhad, Amir
• 金额: $ 2.84万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=759773
• 关键词: Next; generation; microfluidic; nanosensing; platforms

Importance: Early and accurate detection of many of diseases and injuries is still a challenge due to high cost and limited resolution of diagnostic tools. The current diagnostic methods are mainly based on a combination of clinical presentations and the use of different molecular methods. Most of these detection tools rely on optical and imaging detection systems, which in many cases do not provide conclusive results and are labor intensive, time consuming and expensive, therefore they have not been translated into clinical settings. Moreover, countless toxic chemicals and pollutants are released into the environment, which are not detectable reliably using existing monitoring and sensing systems. Electrochemical sensors have recently found reputation for detection of various molecules due to their highly sensitive and selective performance, rapid, label-free, simple, and ease of automation. However, electrochemical sensors are still expensive and less available, and their modification is costly and time-consuming with a limited reproducibility. Also the utility of these sensors for on-site monitoring and point-of-care settings is still restricted due to manual process of several steps of sensing particularly for simultenous detection of multiple targets. Objective: The goal of the proposed research program is to develop new electrochemical sensing platforms and devices for automatic isolation, purification and ultrasensitive detection of various biomolecules, bioparticles, and environmental toxins and pollutants. Anticipated Outcome: Our sensing devices are expected to address the unmet needs of rapid and accurate diagnostics and environmental monitoring via highly sensitive and selective detection of different target molecules and particles in low-cost and automated sensing systems. These devices present new detection methods that (1) do not require dilution of  samples; (2) need a small volume of biofluids, (3) maintain the viability of target particles; (4) minimize the number of processing steps and time between sample collection and target detection; and (5) have the capacity of detecting multiple target molecules and particles simultaneously. Benefits to Canada: This project will train the next generation of researchers with the interdisciplinary expertise in nanotechnology, diagnostic tools, and sensing devices. It will also lead to economic benefits for Canada through developing nanomaterials and sensing tools, launching new spin-off companies for medical diagnostic and environmental monitoring tools, and creating demands for Canada's nanotechnology and diagnostic products. The sensing products will serve Canadian patients by enabling them to receive the most appropriate therapy at an earlier time-point than previously possible and support the development of efficient strategies for environmental protection.

重要性：由于诊断工具的成本高和分辨率有限，早期、准确地检测许多疾病和损伤仍然是一个挑战。目前的诊断方法主要基于临床表现的结合和不同分子方法的使用。这些检测工具依赖于光学和成像检测系统，在许多情况下不能提供结论性的结果，并且是劳动密集型、耗时且昂贵的，因此它们尚未转化为临床环境，此外，无数的有毒化学物质和污染物被释放到环境中。无法可靠检测的环境使用现有的监测和传感系统，电化学传感器最近因其高灵敏度和选择性性能、快速、无标记、简单且易于自动化而在检测各种分子方面享有盛誉。 ，并且它们的修改成本高昂且耗时，并且再现性有限。此外，由于多个感测步骤的手动过程，特别是同时检测，这些传感器在现场监测和护理点设置中的实用性仍然受到限制。目标：多个目标。拟议的研究计划是开发新的电化学传感平台和设备，用于自动分离、纯化和超灵敏检测各种生物分子、生物颗粒以及环境毒素和污染物。 预期结果：我们的传感设备有望解决快速准确诊断的未满足需求。这些设备提供了新的检测方法，（1）不需要稀释样品；（2）通过对不同目标分子和颗粒进行高灵敏度和选择性检测来进行环境监测。体积小，(3) 保持目标颗粒的活力；(4) 最大限度地减少样品采集和目标检测之间的处理步骤数量和时间；(5) 具有同时检测多个目标分子和颗粒的能力。加拿大：该项目将培训具有纳米技术、诊断工具和传感设备跨学科专业知识的下一代研究人员，还将通过开发纳米材料和传感工具、创办新的医疗衍生公司为加拿大带来经济效益。诊断和环境监测工具，并创造需求加拿大的纳米技术和诊断产品将为加拿大患者提供服务，使他们能够比以前更早地接受最合适的治疗，并支持制定有效的环境保护战略。