Droplet-PCR point-of-care device for rapid detection of SARS-CoV-2 and differentiation from influenza

用于快速检测 SARS-CoV-2 并区分流感的液滴 PCR 床旁设备

• 批准号: 561767-2021
• 负责人: SanatiNezhad, Amir
• 金额: $ 1.46万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=719118
• 关键词: Droplet; PCR; point; care; device

The gold standard method for the diagnosis of viral infections is real-time reverse transcription Polymeric Chain Reaction (rRT-PCR). However, almost all rRT-PCR tests need to be done by skilled personnel in the lab setting and with expensive devices, making it far from a point-of-care (PoC) devices to enable remote- and home-based PCR testing. There is a pressing need for the next generation of PCR-based detection assays that not only are able to perform rapid, very accurate, and for PoC and home-based uses for detection of SARS-CoV-2 but also capable of differentiating among different viral and bacterial infections, necessitating the need to better multiplexing and multi-target testing.In the partnership with Accumol in this project, we will use integrated digital droplet microfluidics and microelectronic systems to develop a low-cost, portable, autonomous, and highly accurate droplet PCR (d-PCR) device that can detect SARS-CoV-2 and differentiate it from other viral infections. The deliverable of this project would be to 1) produce functional RNA amplification PoC device-specific to target SARS-CoV-2 RNAs; 2) develop d-PCR protocols useful for selective differentiation of COVID-19 from other viral infections; 3) increase intellectual property in this area and leverage the background patents on distinct categories of IPs, and 4) disseminate technological progress and scientific knowledge through publications in a high-caliber biomedical engineering journal. The d-PCR and detection protocols developed in this project will contribute to implementing new point-of-care testing for rapidly detecting SARS-CoV-2 and differentiate it from other viral infections, addressing the need for Rapid Response to viral infectious disease outbreaks in Canada. One Ph.D. student with a background in biomedical engineering will be trained over the course of the project, directly supported by the requested funding.

诊断病毒感染的金标准方法是实时逆转录聚合链反应（rRT-PCR）。然而，几乎所有 rRT-PCR 测试都需要由技术人员在实验室环境中使用昂贵的设备完成，这使得它远非能够实现远程和家庭 PCR 测试的即时护理 (PoC) 设备。迫切需要下一代基于 PCR 的检测方法，不仅能够快速、非常准确地进行 SARS-CoV-2 的 PoC 和家庭检测，而且能够区分不同的病毒病毒和细菌感染，需要更好的多重和多目标测试。在这个项目中与 Accumol 合作，我们将使用集成数字液滴微流体和微电子系统来开发一种低成本、便携式、自主且高精度的方法液滴PCR (d-PCR) 装置可以检测 SARS-CoV-2 并将其与其他病毒感染区分开来。该项目的可交付成果是 1) 生产针对 SARS-CoV-2 RNA 的功能性 RNA 扩增 PoC 设备； 2) 开发可用于选择性区分 COVID-19 与其他病毒感染的 d-PCR 方案； 3) 增加该领域的知识产权，并利用不同类别知识产权的背景专利，4) 通过在高水平生物医学工程期刊上发表出版物来传播技术进步和科学知识。该项目开发的 d-PCR 和检测方案将有助于实施新的即时检测，以快速检测 SARS-CoV-2 并将其与其他病毒感染区分开来，满足快速应对病毒传染病爆发的需求加拿大。一名博士。具有生物医学工程背景的学生将在项目过程中接受培训，并由所要求的资金直接支持。