Dynamic Light Scattering Instrumentation for Materials Development

用于材料开发的动态光散射仪器

• 批准号: RTI-2023-00419
• 负责人: Chen, Jennifer
• 金额: $ 9.91万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=747818
• 关键词: Dynamic; Light; Scattering; Instrumentation; Materials

A cornerstone analytical technique for characterizing the size and surface charge of colloids is dynamic light scattering (DLS). It is a key missing infrastructure at York University for numerous groups across Chemistry, Engineering and Biology researching on materials for analytical sciences, biomedical tools, vaccine development and environmental monitoring. DLS is the only in situ characterization technique that provides size and charge information of colloids readily. Colloids with different sizes, and charges under an electric potential, diffuse at different rates and yield random fluctuations in the scattering signal. Quantitative information, such as the hydrodynamic radius, zeta potential, and particle concentration, can be deduced based on the time scale of these fluctuations via autocorrelation signal processing. As a result, DLS is non-destructive and ideal for characterizing subnanometer to micron-sized particles in different solvents with minimal sample requirement. It is the only suitable equipment to address our needs in characterizing a diverse range of materials, from inorganic nanoparticles to polymers, lipid vesicles, microspheres and microplastics. We urgently need this equipment because our research, both in fundamental scientific inquiry and industrial collaborations, is facing bottlenecks and inefficiencies as we rely on qualitative observations instead of quantitative information. We often must proceed with experiments without verification of the targeted colloidal properties or biomolecular interactions, and we are only able to detect a problem downstream at a much later stage. The NSERC RTI will enable the acquisition of an in-house DLS instrument that will be fully utilized by at least 5 research groups plus numerous collaborators. It will enable equitable access to a fundamental materials characterization technique for all HQP.

一种用于表征胶体大小和表面电荷的基石分析技术是动态光散射（DLS）。这是约克大学（York University）的主要基础设施缺失，针对化学，工程和生物学研究的众多小组，研究了分析科学，生物医学工具，疫苗开发和环境监测的材料。 DLS是唯一可以轻松提供胶体的大小和电荷信息的原位表征技术。具有不同尺寸的胶体，电势下的电荷以不同的速率扩散，并在散射信号中产生随机波动。可以根据自相关信号处理这些波动的时间尺度来推导定量信息，例如流体动力半径，ZETA电位和颗粒浓度。结果，DLS是无损的，非常适合将次纳米表描述到具有最小样品要求的不同溶剂中的微米大小的颗粒。它是唯一适合我们表征各种材料（从无机纳米颗粒到聚合物，脂质囊泡，微球和微塑料）需求的设备。我们急切需要这种设备，因为我们的研究在基本的科学探究和工业合作中都面临着瓶颈和效率低下，因为我们依靠定性观察而不是定量信息。我们通常必须进行实验，而无需验证靶向胶体特性或生物分子相互作用，并且我们只能在以后的阶段检测到下游的问题。 NSERC RTI将使您能够收购内部DLS仪器，该工具将被至少5个研究小组以及众多合作者全面利用。它将能够公平地访问所有HQP的基本材料表征技术。