Biomolecular-Mediated Plasmonic Sensors for Chemical and Biosensing in Complex Media

用于复杂介质中化学和生物传感的生物分子介导的等离子体传感器

基本信息

批准号：
435664-2013
负责人：
Chen, Jennifer
金额：
$ 2.55万
依托单位：
York University
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2019
资助国家：
加拿大
起止时间：
2019-01-01 至 2020-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=685457
关键词：
Biomolecular Mediated Plasmonic Sensors Chemical

项目摘要

Chemical and biological sensors are important in diverse fields including medical biodiagnostics, national security, environmental protection and agriculture quality control. A range of sensing techniques has been explored but one shortcoming of many sensing platforms is the false positive response from interfering species. Interference in an assay is most commonly reduced by purification processes or by employing surface modifications that prevent non-specific binding interactions. New approach to address this challenge can decrease the cost and complexity of the sensor, shorten the time of analysis and circumvent the need of synthetically demanding chemicals for providing non-fouling surface properties. Our research aims to achieve designed optical response that will differentiate between target analyte from interfering molecules by integrating conformational adaptive biomolecules with plasmonic nanomaterials. The optical properties will arise from the coupling interaction of closely-linked nanoparticles, and changes in the shapes and sizes of the nanoparticles. Importantly, discriminating between target binding and non-specific adsorption of interfering species will be possible because the optical signals generated by the competing processes will be physically different. By exploring engineered biomolecules that can bind to a variety of analytes, these optically active bio-nanomaterials will be developed for screening potential drug molecules, monitoring local chemical environment in cellular processes, and as economical portable diagnostics for on-site field-based detections.

化学和生物传感器在包括医学生物诊断，国家安全，环境保护和农业质量控制在内的不同领域很重要。已经探索了一系列感应技术，但是许多传感平台的一个缺点是干扰物种的假阳性反应。通过纯化过程或采用防止非特异性结合相互作用的表面修饰来减少分析的干扰。应对这一挑战的新方法可以降低传感器的成本和复杂性，缩短分析时间，并规避需要合成的化学品以提供非烧结表面特性。我们的研究旨在实现设计的光学响应，通过将构象自适应生物分子与等离激元纳米材料的构象自适应生物分子进行区分，从而将目标分析物与干扰分子区分开。光学特性将来自紧密连接的纳米颗粒的耦合相互作用，以及纳米颗粒的形状和大小的变化。重要的是，可以区分靶标结合和干涉物种的非特异性吸附，因为竞争过程产生的光信号在物理上会不同。通过探索可以与各种分析物结合的工程生物分子，将开发这些光活性的生物纳米材料，以筛选潜在的药物分子，监测细胞过程中的局部化学环境以及作为现场现场检测的经济便携式诊断。