High speed, area CMOS camera for phase stable imaging and Computational Adaptive Optics

用于相位稳定成像和计算自适应光学的高速面阵 CMOS 相机

• 批准号: RTI-2021-00780
• 负责人: Bizheva, Kostadinka
• 金额: $ 7.16万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718784
• 关键词: speed; area; CMOS; camera; phase

Optical methods that can image the structure and blood flow of biological tissue and probe its normal / abnormal function in-vivo, non-invasively and with cellular-level resolution, can be used clinically for real-time, non-contact optical (virtual) biopsy. As such, they have the potential to revolutionize the early diagnostics and to aid the treatment (surgical and non-surgical) of many diseases, from potentially blinding neurodegenerative retinal diseases to various types of cancer. Optical Coherence Tomography (OCT) is an imaging modality that can image in-vivo, non-invasively and simultaneously the cellular structure, blood flow and physiological function of biological tissue. However, the OCT images suffer from optical aberrations such as defocus, astigmatism and chromatic aberrations, which cause image distortions and blur. When applied to OCT images, an image processing algorithm named Computational Adaptive Optics (CAO), can compensate numerically these aberrations. Processing OCT images with CAO requires that the images are free of motion artefacts. Heart rate, breathing rate and involuntary muscle twitching which occur at frequencies of ~0.5Hz to ~3Hz, can introduce motion artefacts in the images if the OCT image acquisition rate is comparable to or slower than these motions. Current OCT systems use linear array cameras with frame rates < 200 fps, therefore the volumetric OCT imaging rate is < 0.2 Hz. Recently, our research group has developed OCT technology based on an ultrafast area camera (~2,500 fps) that can achieve volumetric imaging rate of ~2.5Hz which can suppress some, though not all motion artefacts and especially fast eye motion. Here, we propose to replace the old camera with a new one which offers a frame rate of 4,500 fps, sufficiently high to suppress effectively motion-induced image artefacts and therefore allow for processing of the OCT images with CAO. The new ultrafast camera provides optimal combination of pixel resolution (2560 x 1920), fast frame rate (4,500 fps) and low noise. The camera can be integrated easily into a fully operational OCT system already available in our research lab and the integration will require minimal hardware and software modifications of the current OCT system. As an integral part of the OCT system, the new camera will be utilized for a number of collaborative research projects focusing on fundamental and applied biomedical research of various diseases such as potentially blinding ocular pathologies (age related macular degeneration, diabetic retinopathy, glaucoma, etc.), neurodegenerative conditions (Alzheimer's, etc.) and various types of cancer. Students working on this research will benefit from research that is made more competitive through the addition of the new camera to the OCT system and the capabilities it will provide. It will enable their work to be published in high-impact factor journals such as “Nature Biophotonics” and Light Science and Applications”.

可以对生物组织的结构和血流进行成像并在体内探测其正常 /异常功能的光学方法，非侵入性和与细胞水平分辨率进行临床用于实时，非接触式光学（虚拟）活检。因此，他们有可能改变早期诊断，并帮助许多疾病的治疗（手术和非手术），从潜在盲目的神经退行性残留疾病到各种类型的癌症。光学相干断层扫描（OCT）是一种成像方式，可以在体内形象，非侵入性，仅仅是生物组织的细胞结构，血流和身体功能。但是，OCT图像遭受光学畸变，例如散焦，散光和色差，这些畸变会导致图像畸变和模糊。当应用于OCT图像时，称为计算自适应光学器件（CAO）的图像处理算法可以通过数值来补偿这些畸变。 使用CAO处理OCT图像需要图像没有运动文物。如果OCT图像采集率与这些动作相当或较慢，则以〜0.5Hz至〜3Hz的频率发生的心率，呼吸速率和非自愿肌肉抽搐会引入图像中的运动伪像。当前的OCT系统使用帧速率<200 fps的线性阵列摄像机，因此体积OCT成像速率为<0.2 Hz。 最近，我们的研究小组基于超快区域摄像头（约2500 fps）开发了OCT技术，该技术可以达到〜2.5Hz的体积成像速率，这可以抑制一些，尽管并非全部运动伪像，尤其是快速的眼睛运动。在这里，我们建议将旧相机替换为新相机，该摄像头提供4,500 fps的帧速率，足够高以抑制有效运动诱导的图像人工制品，因此可以使用CAO处理OCT图像。新的Ultrafast摄像头可提供像素分辨率（2560 x 1920），快速帧速率（4,500 fps）和低噪声的最佳组合。可以轻松地将摄像机集成到我们的研究实验室中已经可用的完全操作的OCT系统中，并且集成将需要对当前OCT系统的最小硬件和软件修改。 作为OCT系统不可或缺的一部分，新相机将用于许多协作研究项目，重点介绍各种疾病的基本和应用生物医学研究，例如潜在盲目的眼科病理学（与年龄相关的黄斑变性，糖尿病性视网膜病变，糖尿病性视网膜病变，glacoma等），神经变性疾病（Alzheimerers等）和各种类型。 从事这项研究的学生将受益于通过将新相机添加到OCT系统及其将提供的功能的研究中更具竞争力的研究。这将使他们的作品能够在“自然生物探测器”以及光学科学和应用等高影响力因素期刊上发表。