Deformable Mirror for Adaptive Optics OCT

用于自适应光学 OCT 的变形镜

• 批准号: RTI-2022-00169
• 负责人: Bizheva, Kostadinka
• 金额: $ 5.36万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=762932
• 关键词: Deformable; Mirror; Adaptive; Optics; OCT

Optical methods that can image the structure and blood flow of biological tissue and probe its normal / abnormal function in-vivo, non-invasively and with cellular-level resolution, can be used clinically for real-time, non-contact optical (virtual) biopsy. As such, they have the potential to revolutionize the early diagnostics and to aid the treatment (surgical and non-surgical) of many diseases, from potentially blinding neurodegenerative retinal diseases to various types of cancer. Optical Coherence Tomography (OCT) is an imaging modality that can image in-vivo, non-invasively and simultaneously the cellular structure, blood flow and physiological function of biological tissue. However, the OCT images suffer from optical aberrations such as defocus, astigmatism and chromatic aberrations, which cause image distortions and blur. Adaptive Optics (AO) can measure the wavefront aberrations and correct for them in the detected optical beam, thus generating high resolution, aberrations-free images of the imaged object. AO can be combined with other optical imaging modalities such as OCT, to improve their enaface spatial resolution. Recently, our research group has built a Line-Scan-OCT (LS-OCT) system that utilizes an ultrahigh speed camera and can acquire volumetric images of biological tissue at imaging rates of 5,000 fps or higher, while providing cellular level resolution of ~ 2 µm. Here, we propose to upgrade the LS-OCT system with an AO arm, to allow for correcting of aberrations in the LS-OCT images. To upgrade the LS-OCT system we require funds to purchase 2 components: a deformable mirror for correcting the aberrations in the OCT imaging beam; and a broad bandwidth light source to replace the laser that was used to power the LS-OCT and which recently broke down and is beyond repair. Students working on this research will benefit from research that is made more competitive through the development of the new AO-LS-OCT system and the capabilities it will provide. It will enable their work to be published in high-impact factor journals such as "Nature Biophotonics" and Light Science and Applications".

可以对生物组织的结构和血流进行成像并在体内探测其正常 /异常功能的光学方法，非侵入性和与细胞水平分辨率进行临床用于实时，非接触式光学（虚拟）活检。因此，他们有可能改变早期诊断，并帮助许多疾病的治疗（手术和非手术），从潜在盲目的神经退行性残留疾病到各种类型的癌症。光学相干断层扫描（OCT）是一种成像方式，可以在体内形象，非侵入性，仅仅是生物组织的细胞结构，血流和身体功能。但是，OCT图像遭受光学畸变，例如散焦，散光和色差，这些畸变会导致图像畸变和模糊。自适应光学元件（AO）可以测量波前畸变并在检测到的光束中校正它们，从而产生高分辨率，无像差的对象的畸变图像。 AO可以与其他光学成像方式（例如OCT）结合使用，以改善其启用空间分辨率。最近，我们的研究小组建立了一个使用超速度摄像头的线扫描-OCT（LS-OCT）系统，可以以5,000 fps或更高的成像速率获得生物组织的体积图像，同时提供约2 µm的细胞水平分辨率。在这里，我们建议使用AO ARM升级LS-OCT系统，以纠正LS-OCT图像中的像差。为了升级LS-OCT系统，我们需要资金购买2个组件：可变形的镜子，用于校正OCT成像光束中的像差；以及一个宽阔的带宽光源，以取代用于为LS-OCT供电的激光器，并且最近破裂并无法修复。从事这项研究的学生将受益于通过开发新的AO-LS-OCT系统及其将提供的功能，从而使研究更具竞争力。这将使他们的工作能够发表在“自然生物生物原理”以及光学科学与应用等高影响力因素期刊上。