1000 individually-controlled microcosms to assess ecological impacts in Canadian ecosystems, aquaculture, and agriculture

1000 个单独控制的微观世界，用于评估加拿大生态系统、水产养殖和农业的生态影响

• 批准号: 506882-2017
• 负责人: Sinton, David
• 金额: $ 15.95万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=694612
• 关键词: 1000; individually; controlled; microcosms; assess

Canadian aquaculture and agriculture will be subject to a complex mix of global change in combination with local stressors. With our combined engineering-ecological expertise we will develop and apply a platform for measuring organism and community responses to multiple environmental parameters. This work is enabled by the novel concept of an aerogel-based gas gradient generator that achieves unprecedented control of local CO2 and O2 concentration over microwells. This gas control technology will be combined with projection optics and microwell plate-based liquid handling to enable experiments with 1000+ parallel treatments for full-factorial data sets with a wide spectrum of characteristically different parameters. The 3-year deliverable is a commercialization-ready screening platform capable of quantifying the response of model microorganisms, flora, and fauna to key environmental variables. The project provides data directly relevant to Canadian aquaculture (Nova Harvest) and agriculture (POS Biosciences) and leverages Canadian product development excellence (Quorum Technologies). Both the method and the data produced through this project directly address the "Environment and Agriculture" target area. Specifically, we will qualify the impact of global and local anthropogenic stressors on the aquatic environment. Moreover, the technology will equip a broad range of Canadian companies to adapt agriculture and aquaculture for a changing climate. In close partnership with Canadian leaders aquaculture (Nova Harvest), agriculture (POS Biosciences), and device development (Quorum Technologies), this project will provide both industry-ready screening technology, and develop outstanding HQP with new tools to assess climate change impacts in Canada and worldwide.

加拿大的水产养殖和农业将与当地压力源相结合的全球变化结合。借助我们合并的工程生态专业知识，我们将开发并应用一个平台来衡量有机体和社区对多个环境参数的反应。这项工作是通过基于Aerogel的气体梯度发生器的新型概念来实现的，该概念可以实现对微小细胞上局部CO2和O2浓度的前所未有的控制。该气体控制技术将与投影光学和基于微板板的液体处理结合使用，以实现1000多次平行处理的实验，以供具有广泛特征性不同参数的全因素数据集。为期3年的可交付方式是一个可以进行商业化的筛选平台，能够量化模型微生物，动植物对关键环境变量的响应。 该项目提供了与加拿大水产养殖（Nova Harvest）和农业（POS Biosciences）和杠杆加拿大产品开发卓越（Quorum Technologies）直接相关的数据。通过该项目产生的方法和数据都直接解决了“环境和农业”目标领域。 具体而言，我们将鉴定全球和局部人为应激源对水生环境的影响。此外，该技术将配备广泛的加拿大公司，以适应农业和水产养殖的气候变化。与加拿大领导人水产养殖（Nova Harvest），农业（POS Biosciences）和设备开发（法定技术）密切合作，该项目将既可以提供适合行业的筛查技术，并开发出出色的HQP，并使用新的工具来评估加拿大和全球范围内的气候变化影响。