Electrochemical drug sensing with hydrophobic compound-adapted aptamers

使用疏水性化合物适配适配体进行电化学药物传感

• 批准号: 521437-2018
• 负责人: Perreault, Jonathan
• 金额: $ 14.35万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=694154
• 关键词: Electrochemical; drug; sensing; hydrophobic; compound

Drug usage is responsible for numerous adverse effects on public health and safety (overdoses, car accidents, crime...). Accordingly, simple and rapid detection of illicit drug smuggling and of drug usage during driving is of paramount importance. The latter is particularly relevant in the context of the expected 2018 marijuana legalization, accompanied with a so-called "zero-tolerance while driving" policy for which appropriate detection-technology is not currently available. Detection often relies on non-optimal targets. For example, studies indicate that to evaluate driving impairment induced by cannabis, it would be more appropriate to use a ratio of THC (the main active ingredient of marijuana) and metabolized THC molecules. In parallel, volatile derivatives of cocaine are more useful for detection of drug smuggling. Most of the current portable technologies use antibodies, which do not discern THC from its metabolites and do not bind to the most volatile compounds derived from drugs (like cocaine derivatives). The hydrophobicity of these drugs and their derivatives poses technical problems to find biosensing-molecules that recognize them. We plan to overcome this challenge with modified aptamers (which are DNA molecules that recognize target analytes with a tailored selectivity) combined to an electrochemical platform for transduction of the binding event. The proposed advances are expected to lead to biosensors for simple, cheap, ultrasensitive and rapid drug detection which will help counter drug smuggling and provide much needed tools for cannabis testing for drivers. The partnership with the two companies involved and the team put together to achieve these goals combines high level expertise and infrastructures that will provide an outstanding environment for the training of students and PDFs. In addition, it will contribute to keep Canada at the forefront of aptamer and biosensing research as well as provide technologies to solve very current problematics in Canada.

药物使用造成对公共卫生和安全的无数不利影响（过量，汽车事故，犯罪...）。因此，在驾驶过程中对非法毒品走私和药物使用的简单和快速检测至关重要。后者在预期的2018年大麻合法化的背景下特别重要，并伴随着所谓的“零容忍”政策，目前尚无适当的检测技术。检测通常依赖于非最佳目标。例如，研究表明，为了评估大麻引起的驾驶障碍，使用THC的比率（大麻的主要活性成分）和代谢的THC分子更合适。同时，可卡因的挥发性衍生物对于检测毒品走私更有用。 当前的大多数便携式技术都使用抗体，这些抗体并不能与其代谢产物辨别，并且不与来自药物（例如可卡因衍生物）得出的最挥发性化合物结合。这些药物及其衍生物的疏水性提出了技术问题，可以找到识别它们的生物传感分子。我们计划通过修改的适体（这些分子识别具有量身定制的选择性的目标分析物的DNA分子）来克服这一挑战，将其结合到电化学平台上，以转导结合事件。拟议的进步预计将导致生物传感器以简单，廉价，超敏感和快速的药物检测为生物传感器，这将有助于对抗毒品走私，并为驾驶员提供大麻测试的急需工具。 与相关的两家公司和团队共同实现这些目标的合作伙伴关系结合了高级专业知识和基础架构，为学生和PDF的培训提供了杰出的环境。此外，它将有助于使加拿大保持适体和生物传感研究的最前沿，并提供技术来解决加拿大非常当前的问题。