Sensitive and specific electrochemical detection of COVID-19 (SARS-CoV2) based on isothermal amplification

基于等温扩增的 COVID-19 (SARS-CoV2) 的灵敏且特异的电化学检测

• 批准号: 554464-2020
• 负责人: Perreault, Jonathan
• 金额: $ 3.64万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=705425
• 关键词: Sensitive; specific; electrochemical; detection; COVID

The epidemic of the SARS-CoV2 virus (COVID-19) and its global spread urgently requires efficient means for rapid diagnosis, as well as detection in various environments (aside from clinical laboratories), such as airplanes, schools, potential animal carriers, etc. Indeed, most of the countries that appear to go through the current pandemic with currently relatively low death tolls and that are managing partial deconfinement are among those that are doing the most virus tests. We have previously devised a colorimetric method to detect specific virus DNA which could potentially be used for "on site" virus detection and which, in combination with an electrode-based detection device, would provide simple, sensitive and cheap on-site detection of the virus. SARS-CoV2 (the pathogen causing COVID-19) is an RNA virus, which adds a step for its amplification with the most commonly used detection methods, compared to other viruses with a genome made of DNA. The method we want to implement detects RNA and DNA in a similar way. Moreover, the current gold standard for virus detection requires specialized laboratory material and personnel, which also means sample transportation logistics and waiting queues for samples to be processed. We aim at implementing a novel DNA amplification method (called Rolling Circle Amplification) which does not rely on specialized equipment. By combining this with electrodes we expect to achieve sensitive and rapid detection of the virus genome without the need for bulky complex devices. Even if we all hope for a vaccine to come as soon as possible, there is still a dire need for an efficient, rapid and simple test to monitor, perhaps even on a daily or weekly basis, everyone who is going back to work or school to ensure that we can quickly respond to any new eclosion as soon as it can be detected. This would allow to have confinement measures targeted only on the workplaces with acknowledged COVID-19, but without affecting all work places and schools simultaneously.

SARS-COV2病毒（Covid-19）的流行及其全球传播及其全球蔓延需要有效的手段来快速诊断，以及在各种环境（除了临床实验室）中的检测，例如飞机，学校，潜在的动物载体等。的确，这些国家的大多数国家似乎都在当前具有相对低位的死亡人数，而这些国家似乎都在这些国家中，这些国家都处于这种状态，而这些国家的死亡人数均已占有一致，而这些国家都在这种情况下，而这些国家都在这种情况下，这些国家均具有一致性。测试。我们以前已经设计了一种检测特定病毒DNA的比色方法，该方法可能用于“现场”病毒检测，并与基于电极的检测装置结合使用，可以提供简单，敏感且廉价的现场检测病毒。 SARS-COV2（引起Covid-19的病原体）是RNA病毒，与其他具有DNA基因组的病毒相比，它使用最常用的检测方法增加了其扩增的步骤。我们想要实现的方法以类似的方式检测RNA和DNA。此外，当前的病毒检测金标准需要专门的实验室材料和人员，这也意味着样品运输物流和等待队列以处理样品。我们旨在实施一种新型的DNA扩增方法（称为滚动圆扩增），该方法不依赖专用设备。通过将其与电极相结合，我们期望在不需要笨重的复合装置的情况下实现病毒基因组的敏感和快速检测。 即使我们所有人都希望尽快采用疫苗，仍然需要进行高效，快速和简单的测试来监控，甚至可能每天或每周一次，也可能会重新上班或学校，以确保我们能够在检测到任何新的eClosion时可以快速响应任何新的Eclosion。这将允许仅针对公认的Covid-19的工作场所采取限制措施，但不会同时影响所有工作场所和学校。