A confocal laser scanning microscope for innovative research in animals, plants, and food science

用于动物、植物和食品科学创新研究的共焦激光扫描显微镜

• 批准号: RTI-2020-00095
• 负责人: Guan, Leluo
• 金额: $ 10.92万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693699
• 关键词: confocal; laser; scanning; microscope; innovative

We are requesting funds to acquire a confocal laser scanning microscope, which will be of immense benefit to a team of internationally reputed team leaders in their multidisciplinary research programs. All the applicants (6) and collaborators (5) on this proposal are conducting research in the areas of animal, plant, and food science. Some of the major areas of investigation by these researchers are to investigate: the biodiversity and biofunctions of bovine gut microflora; host regulatory gene and protein markers in complex traits in cattle and swine; composition of undigested residue in relation to gut microbiome in swine; metagenomic analysis of bacterial populations in intestinal tissues in piglets; the mechanisms of action of bioactive peptides in cells and animal tissues; plant insect and plant-fungal interactions for best management strategies; and characteristics of muscle and adipose tissue related to cancer-associated myosteatosis. For all of these discovery-based and applied research projects; direct visualization and imaging method using confocal laser scanning microscope is a fundamental requirement. Availability of this microscope will promote research and innovations with a major impact in the areas of agricultural production, food quality, animal and human health in beef, dairy and swine industries, knowledge-based disease management in plants, develop functional food ingredients and biomaterials for biomedical applications. This equipment will be housed in a multi-user core genomics facility in the Department of Agricultural, Food and Nutritional Science, which has provided training to over 750 highly qualified personnel in the last 10 years on genomics and other related instrumentation. Currently, graduate, undergraduate, summer students, postdoctoral fellows, research associates and technologists use this facility, receive training and integrate these high-throughput genomics and molecular technologies in their research projects. The work to be facilitated by the confocal microscope will play an important role in the advancement of the currently funded research projects of the applicants and collaborators on this proposal and will promote new partnerships. Furthermore, the availability of the confocal microscope will be critical for advancement of our knowledge for agricultural discovery and innovation and prepare highly qualified personnel for industry and academia.

我们要求资金购买共聚焦激光扫描显微镜，这将对其多学科研究计划中的国际知名团队负责人团队带来巨大好处。所有申请人（6）和合作者（5）在该提案中正在对动物，植物和食品科学领域进行研究。 这些研究人员的一些主要调查领域是研究：牛肠道微生物的生物多样性和生物功能；牛和猪中复杂性状的宿主调节基因和蛋白质标记；与猪中肠道微生物组有关的未消化残留物组成；小猪肠道组织细菌种群的宏基因组分析；生物活性肽在细胞和动物组织中的作用机制；最佳管理策略的植物昆虫和植物恋爱相互作用；肌肉和脂肪组织的特征与与癌症相关的肌toposisis有关。对于所有这些基于发现和应用的研究项目；使用共聚焦激光扫描显微镜的直接可视化和成像方法是基本要求。该显微镜的可用性将促进研究和创新，对牛肉，奶制品和猪行业的农业生产，粮食质量，动物和人类健康，植物中基于知识的疾病管理，开发功能性食品成分和生物材料的研究和创新。生物医学应用。该设备将安置在农业，食品和营养科学系的多用户核心基因组学设施中，该机构在过去的10年中为基因组学和其他相关仪器提供了750多名高素质人员的培训。目前，研究生，本科生，夏季学生，博士后研究员，研究助理和技术人员使用此设施，接受培训并将这些高通量基因组学和分子技术整合到其研究项目中。共聚焦显微镜将促进的工作将在当前资助的申请人和合作者在该提案上的研究项目中发挥重要作用，并将促进新的合作伙伴关系。此外，共聚焦显微镜的可用性对于提高我们的农业发现和创新知识并为工业和学术界准备高素质的人员至关重要。