Pyrosequencer for genomics and epigenomics research

用于基因组学和表观基因组学研究的焦磷酸测序仪

• 批准号: RTI-2017-00400
• 负责人: Guan, Leluo
• 金额: $ 7.52万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616665
• 关键词: Pyrosequencer; genomics; epigenomics; research

A multi-user core facility, Agricultural Genomics and Proteomics Unit (AGP Unit), in the Department of Agricultural, Food and Nutritional Science, provides key capacity for researchers employing genomics technologies for technological innovation in food and the bio-economy. These internationally reputed multidisciplinary research programs include The Livestock Gentec (bovine, swine, chicken), meat science and processing, swine nutrition, reproductive physiology, rangeland ecology and management, and collaborating scientists at Agriculture and Agri-Food Canada. Some of the major areas of investigation by these researchers are focused on improving the understanding of genetic and epigenetic changes, impacting complex traits such as feed efficiency, animal and plant health. The epigenetics modifications result in variations of gene expression without any change in the DNA sequence (e.g., the methylation of cytosine, methylation, acetylation etc.). These include feed efficiency, immune response, microbial-host interactions, the role of gut microflora in beef, dairy cattle and pigs related to animal health and meat quality, genetic background/environment associated with microbiome; early-life nutrition and management programs in dairy cows, epigenome of the pancreas, rangeland ecology, biodiversity studies, impact of production practices on meat quality and providing better tools for genetic/epigenetic improvement and gene-assisted management. It is necessary to acquire a pyrosequencer for the AGP unit researchers, to identify the functional single nucleotide polymorphism and epigenetic markers, in addition to the existing genomics and functional genomics researches. The requested Pyrosequencer will be providing further knowledge in genomic response to environment and diet using epigenomics to develop coordinated nutritional and management strategies related to animal production, health, crop sciences, food science and safety, and agri-business. These studies will enhance the development of tools that identify specific performance, quality traits, and value added characteristics in animal and plant systems and facilitate training of highly qualified personnel for industry and academia.

农业、食品和营养科学系的农业基因组学和蛋白质组学单位（AGP单位）是一个多用户核心设施，为研究人员利用基因组技术进行食品和生物经济技术创新提供关键能力。这些国际知名的多学科研究项目包括畜牧 Gentec（牛、猪、鸡）、肉类科学和加工、猪营养、生殖生理学、牧场生态和管理，以及加拿大农业和农业食品部的合作科学家。这些研究人员的一些主要研究领域集中于提高对遗传和表观遗传变化的理解，这些变化影响饲料效率、动植物健康等复杂性状。表观遗传学修饰导致基因表达的变化，但 DNA 序列没有任何变化（例如胞嘧啶甲基化、甲基化、乙酰化等）。这些包括饲料效率、免疫反应、微生物与宿主的相互作用、牛肉、奶牛和猪肠道微生物群与动物健康和肉类质量相关的作用、与微生物组相关的遗传背景/环境；奶牛生命早期营养和管理计划、胰腺表观基因组、牧场生态学、生物多样性研究、生产实践对肉质的影响以及为遗传/表观遗传改良和基因辅助管理提供更好的工具。除了现有的基因组学和功能基因组学研究之外，AGP单位的研究人员还需要购买焦磷酸测序仪，以鉴定功能性单核苷酸多态性和表观遗传标记。所要求的焦磷酸测序仪将利用表观基因组学提供对环境和饮食的基因组反应的进一步知识，以制定与动物生产、健康、作物科学、食品科学和安全以及农业企业相关的协调营养和管理策略。这些研究将加强识别动植物系统的具体性能、质量特征和增值特征的工具的开发，并促进工业界和学术界高素质人才的培训。