Photo-Polymerization 3D Printer

光聚合3D打印机

• 批准号: RTI-2019-00293
• 负责人: MoranMirabal, Jose
• 金额: $ 9.14万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693397
• 关键词: Photo; Polymerization; 3D; Printer

3D printing is revolutionizing the way products are manufactured, with benefits including higher customization, expedited prototyping, and lower costs. In particular, the area of biofabrication is expanding rapidly, with the number of publications using 3D printing to design microenvironments for tissue engineering growing exponentially. This proposal seeks funding for a 3D printer capable of photo-polymerization of polymer, hydrogel, and nanomaterial-based bioinks into three-dimensional scaffolds for cell culture and tissue engineering. The requested 3D printer is urgently needed by the Moran-Mirabal, Bowdish, and Didar groups to perform testing and validation of novel green 3D printing bioinks which are based on functional green nanomaterials (Moran-Mirabal), to fabricate scaffolds to study age-related effects on macrophage mobility and their impact on immunity, infection and disease (Bowdish), and to develop new Organ-on-Chip systems for drug screening (Didar). Currently, there are no photo-polymerization 3D printers available at McMaster University, which severely limits our ability to carry out this research, file IP for new materials produced, and publish our findings - ultimately delaying HQP progression. The specific 3D printer requested is capable of printing up to 6 different materials in series, under temperature-controlled conditions, is capable of irradiation in UV and visible wavelengths, and has a small foot print that makes it possible to place it in a biological safety cabinet to print under a sterile environment. The 3D printer will be located in the newly renovated research wing of the Arthur N. Bourns Building at McMaster University, where it will be set up in a Biosafety Level 2 laboratory shared by faculty from the Chemistry and Chemical Biology and Chemical Engineering departments, and will be managed by a laboratory research manager. As such, the equipment will be operated as a shared instrument available to research groups interested in 3D printing scaffolds for biomedical applications. Thus, the availability of such a 3D printer will enhance the research programs not only of the applicants, but also of other groups, including the Wylie, Brook, Adronov, Selvaganapathy, Zhang, Hoare and Grandfield, by allowing them to test new materials (e.g. silicones, hydrogels, polymer bound single walled carbon nanotubes) for 3D printing or evaluate the response of cells to 3D printed scaffolds (e.g., osteoblasts for bone regeneration, mesenchymal stem cells). A delay in securing the requested 3D printer would not only prevent the applicants' research groups from expeditiously moving forward research on testing new green 3D printing bioinks, but also from 3D printing scaffolds to investigate fundamental biological questions. This will ultimately limit our ability to apply for patents, publish our results, and move student research forward to its full potential.

3D 打印正在彻底改变产品的制造方式，其优点包括更高的定制性、更快的原型设计和更低的成本。特别是，生物制造领域正在迅速扩大，使用 3D 打印来设计组织工程微环境的出版物数量呈指数级增长。该提案寻求资助开发一种 3D 打印机，该打印机能够将聚合物、水凝胶和基于纳米材料的生物墨水光聚合成用于细胞培养和组织工程的三维支架。 Moran-Mirabal、Bowdish 和 Didar 小组迫切需要所要求的 3D 打印机，以对基于功能性绿色纳米材料 (Moran-Mirabal) 的新型绿色 3D 打印生物墨水进行测试和验证，以制造支架来研究与年龄相关的对巨噬细胞流动性的影响及其对免疫、感染和疾病的影响 (Bowdish)，并开发用于药物筛选的新芯片器官系统 (Didar)。目前，麦克马斯特大学没有可用的光聚合 3D 打印机，这严重限制了我们进行这项研究、为生产的新材料提交知识产权以及发布我们的研究结果的能力 - 最终推迟了 HQP 的进展。所需的特定 3D 打印机能够在温度控制条件下连续打印多达 6 种不同的材料，能够在紫外线和可见光波长下进行照射，并且占地面积小，因此可以将其放置在生物安全环境中。柜子在无菌环境下打印。这台 3D 打印机将位于麦克马斯特大学 Arthur N. Bourns 大楼新装修的研究楼内，该实验室将设置在由化学、化学生物学和化学工程系的教员共享的生物安全二级实验室中，以及将由实验室研究经理管理。因此，该设备将作为共享仪器运行，供对生物医学应用 3D 打印支架感兴趣的研究小组使用。因此，这种 3D 打印机的可用性不仅将增强申请人的研究计划，而且还将增强其他团体的研究计划，包括 Wylie、Brook、Adronov、Selvaganapathy、Zhang、Hoare 和 Grandfield，让他们能够测试新材料。用于 3D 打印的有机硅、水凝胶、聚合物结合的单壁碳纳米管）或评估细胞对 3D 打印支架（例如用于骨再生的成骨细胞）的反应间充质干细胞）。延迟获得所要求的 3D 打印机不仅会阻碍申请人的研究小组迅速推进测试新型绿色 3D 打印生物墨水的研究，还会阻碍 3D 打印支架研究基本生物学问题。这最终将限制我们申请专利、发布研究成果以及推动学生研究充分发挥潜力的能力。