Rapid, reagent-free technology for identification of methicillin-resistant staphylococcus aureus (MRSA) and vancomycin-resistant entercococci (VRE) in clinical microbiology labs

临床微生物实验室中快速、免试剂鉴定耐甲氧西林金黄色葡萄球菌 (MRSA) 和耐万古霉素肠球菌 (VRE) 的技术

• 批准号: 500176-2016
• 负责人: Ismail, Ashraf
• 金额: $ 8.94万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=607949
• 关键词: Rapid; reagent; free; technology; identification

Infections caused by antibiotic-resistant bacteria are a major concern in hospitals worldwide. Staphylococcal and enterococcal bacteria are major causes of hospital-acquired infections, including half of all bloodstream infections. Canadian prevalence studies have estimated that approximately 4% of hospitalized patients will become infected or colonized with MRSA (methicillin-resistant Staphylococcus aureus), and the prevalence of infections caused by vancomycin-resistant enterococci (VRE) in Canadian hospitals tripled between 2007 and 2013. One of the recommendations in the action plan on antimicrobial resistance put forward by the Federal government in 2015 is research and development of faster and more accurate diagnostic tools that can provide doctors the information they need to accurately diagnose an infection, thereby promoting effective use of antimicrobials. This NSERC-I2I Phase I proposal aims at achieving these goals by developing a new rapid and cost-effective technology for the identification of MRSA and VRE based on "whole-organism fingerprinting" by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. The advantages of the new technology include: (i) minimal sample preparation, (ii) reagent-free analysis, (iii) simple (one-step) protocol, (iv) fast turnaround time (1-2 min), and (v) low cost per test. To our knowledge, there is no other technology currently on the market that accomplishes the discrimination between antibiotic-resistant and antibiotic-susceptible bacteria in a single step without the use of any reagents.

由抗生素耐药细菌引起的感染是全世界医院的主要关注点。葡萄球菌和肠球菌是医院获得性感染的主要原因，占所有血液感染的一半。加拿大流行率研究估计，大约 4% 的住院患者会感染或定植 MRSA（耐甲氧西林金黄色葡萄球菌），2007 年至 2013 年间，加拿大医院中耐万古霉素肠球菌 (VRE) 引起的感染流行率增加了两倍。联邦政府2015年提出的抗菌素耐药性行动计划中的建议之一是研发更快、更准确的诊断工具，为医生提供帮助他们需要准确诊断感染的信息，从而促进抗菌药物的有效使用。 NSERC-I2I 第一阶段提案旨在通过开发一种新的快速且经济高效的技术来实现这些目标，该技术用于基于衰减全反射傅立叶变换红外 (ATR-FTIR) 的“整个生物体指纹识别”来识别 MRSA 和 VRE光谱学。新技术的优点包括：（i）最少的样品制备，（ii）无试剂分析，（iii）简单（一步）方案，（iv）快速周转时间（1-2分钟），以及（v） ）每次测试成本低。据我们所知，目前市场上还没有其他技术可以在不使用任何试剂的情况下一步完成抗生素耐药性和抗生素敏感细菌的区分。