Exploration of new approaches to microbial typing and phenotypic characterization based on Fourier transform infrared and high-resolution magic-angle spinning NMR spectroscopy and the development of active packaging employing natural antimicrobials

基于傅里叶变换红外和高分辨率魔角旋转核磁共振波谱探索微生物分型和表型表征的新方法以及采用天然抗菌剂的活性包装的开发

• 批准号: RGPIN-2019-07277
• 负责人: Ismail, Ashraf
• 金额: $ 2.04万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=739392
• 关键词: Exploration; new; approaches; microbial; typing

The first facet of the proposed Discovery Grant research will be directed toward the exploration of the discriminatory nature between closely related bacterial strains based on differences in their Fourier transform infrared (FTIR) spectra, high-resolution magic angle spinning (HRMAS) NMR and mass spectrometry with a focus on their application to microbial typing, outbreak detection and biomarker discovery. In our work, these capabilities are being exploited to develop rapid methods for identification of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), which are among the most prevalent causes of healthcare-associated infections, and identification of emerging multi-drug resistant clinical yeast isolates such as Candida auris. This work is being conducted through collaborative studies with Health Canada, the Quebec clinical surveillance agency and multiple hospitals across Quebec. With regards to foodborne illness, ongoing efforts, in collaboration with researchers at Health Canada and the Canadian Food Inspection Agency (CFIA), is aimed at studying the effect of genetic variability of pathogenic E. coli pathotypes (associated with foodborne illness) to its virulence by whole genome sequencing and FTIR spectroscopy. Our work initially entails acquisition of a large spectral datasets from of microorganisms for the creation of spectral databases containing large numbers of FTIR, 1H and 31P HRMAS NMR and mass spectrometry (MS) spectra of well-characterized strains and extracting the discriminatory spectral information by applying feature-selection algorithms in conjunction with multivariate statistical analysis techniques. A major objective of the proposed research is to develop new strategies directed toward elucidation of the specific biochemical differences underlying the spectral differences among particular bacterial strains. It is anticipated that the results of the proposed research will advance biomarker discovery not only in our work on microbial identification and classification but also in the rapidly evolving field of microbial typing and may aid in the development of new antibiotics that can treat the rising levels of antibiotic nosocomial infections resulting in hospital outbreaks. The second facet of the proposed research will focus on the exploration of proteins in combination of selected polysaccharides as components of biodegradable films and embedding the films with natural antimicrobials to retard microbial growth of fresh and minimally processed foods. The proposed research focuses on the application of infrared imaging microscopy to study the distribution, migration rates of natural antimicrobials in biodegradable films in relation to enhancing their potential use in active packaging.

拟议的发现赠款研究的第一个方面将针对基于其傅立叶变换红外（FTIR）光谱的差异，高分辨率的魔法旋转（HRMAS）NMR和质谱的差异，将紧密相关的细菌菌株之间的歧视性探索，并将其侧重于将其用于微生物型，探索，探索微生物型探测。 In our work, these capabilities are being exploited to develop rapid methods for identification of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), which are among the most prevalent causes of healthcare-associated infections, and identification of emerging multi-drug resistant clinical yeast分离物，例如念珠菌。这项工作是通过与加拿大卫生部，魁北克临床监视局和魁北克多家医院的合作研究进行的。关于食源性疾病，与加拿大卫生部和加拿大食品检验局（CFIA）合作的持续努力旨在研究致病性大肠杆菌病原体病原体病原体遗传变异性（与食品生物疾病相关的遗传变异性）对整个基因组测序和FTIR光谱法对其病毒性的影响。我们的工作最初需要从微生物中获取大量光谱数据集，以创建含有大量FTIR，1H和31p HRMAS NMR和质谱（MS）光谱的光谱数据库，并通过将歧视性谱图通过分析量进行分析量进行分析，以均匀统计量进行分析。拟议研究的一个主要目的是制定针对特定细菌菌株之间光谱差异的特定生化差异的新策略。预计拟议的研究的结果不仅会在微生物识别和分类方面的研究中提高生物标志物的发现，而且还可以在微生物分类的快速发展领域中提高发现，并可能有助于开发新的抗生素，这些抗生素水平上升，导致医院爆发导致抗生素性抗生素感染。拟议的研究的第二个方面将重点介绍蛋白质作为可生物降解膜的组成部分的组合，并用天然抗微生物剂嵌入膜以推迟新鲜和微型加工食品的微生物生长。拟议的研究重点是应用红外成像显微镜在可生物降解膜中天然抗菌剂的分布，迁移速率，与增强其在主动包装中的潜在用途有关。