Upgrade of Spinning Disk Confocal Microscope

转盘共焦显微镜的升级

• 批准号: RTI-2016-00099
• 负责人: Samuels, AnneLacey
• 金额: $ 10.78万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591343
• 关键词: Upgrade; Spinning; Disk; Confocal; Microscope

The Perkin-Elmer (P-E) Ultraview VoX spinning disk confocal microscope is a workhorse instrument for detecting fluorescent proteins, such as green fluorescent protein (GFP), in living cells. We use the P-E Spinning Disk confocal microscope in studies of how cellulose is made in plant cells, for examining plant cells during growth and development, and for investigating plants' and animals' responses to stress. The current microscope is seven years old, and it is beginning to fall behind international standards due to its outdated camera, broken stage (sample holder), and aging lasers. Because the current microscope is outdated, it cannot be used for live cell imaging for very rapid events. As this workhorse microscope ages, we are increasingly facing problems. In order to collect the data for his Ph.D., and his subsequent Science paper, UBC Botany student Yoshi Watanabe was forced to go to Stanford University to use their spinning disk confocal. Our spinning disk requires upgrades to the laser, the stage, the optics, and the camera. The microscope is housed in, and managed by, the UBC Bioimaging Facility, a campus-wide biological imaging facility. UBC Bioimaging Facility provides top-end light and electron microscopy infrastructure to more than 100 research groups from 25 UBC departments. Upgrades to the spinning disk confocal will benefit many HQP from across campus. There were 30 HQP using the microscope in 2014-2015, from Botany (Samuels, Haughn, Douglas, Kunst, Wasteneys labs), Forestry (Mansfield, Bohlmann labs), Pediatrics (Maxwell lab), Microbiology and Immunology (Hancock lab), Chemistry (Perrin lab), and Zoology (Brauner, Milsom, Auld labs). The many on-going projects that rely on this equipment generate the urgency of this request.

Perkin-Elmer (P-E) Ultraview VoX 转盘共聚焦显微镜是检测活细胞中荧光蛋白（例如绿色荧光蛋白 (GFP)）的主力仪器。我们使用 P-E 转盘共聚焦显微镜来研究植物细胞中纤维素的形成方式、检查生长和发育过程中的植物细胞以及研究植物和动物对压力的反应。目前的显微镜已有七年历史，由于相机过时、载物台（样品架）损坏和激光器老化，它开始落后于国际标准。由于当前的显微镜已经过时，它不能用于非常快速事件的活细胞成像。随着这款主力显微镜的老化，我们面临的问题越来越多。为了收集他的博士学位和随后的科学论文的数据，UBC 植物学学生 Yoshi Watanabe 被迫前往斯坦福大学使用他们的转盘共聚焦。我们的旋转盘需要升级激光器、平台、光学器件和相机。该显微镜位于 UBC 生物成像设施（校园范围内的生物成像设施）内并由其管理。 UBC 生物成像设施为 UBC 25 个系的 100 多个研究小组提供高端光学和电子显微镜基础设施。旋转盘共焦的升级将使整个校园的许多 HQP 受益。 2014-2015 年有 30 个 HQP 使用显微镜，分别来自植物学（Samuels、Haughn、Douglas、Kunst、Wasteneys 实验室）、林业（Mansfield、Bohlmann 实验室）、儿科（Maxwell 实验室）、微生物学和免疫学（Hancock 实验室）、化学（Perrin 实验室）和动物学（Brauner、Milsom、Auld 实验室）。 许多正在进行的项目都依赖于该设备，因此这一要求变得紧迫。