Confocal Laser Scanning Microscope with Spinning Disk Technology

采用转盘技术的共焦激光扫描显微镜

• 批准号: 522417173
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/522417173?language=en
• 关键词: Confocal; Laser; Scanning; Microscope; Spinning; Confocal; Laser; Scanning; Microscope; Spinning

OICE is a centre of excellence (central institute) of FAU and represents the centralised platform for high and ultra-high resolution imaging using light-based technologies for, among other things, living cells and tissues, organoids, intravital imaging and imaging of biological materials and membranes. OICE combines a Core Facility Unit (CFU), Exploratory Research Unit (ERU) and Educational Training Unit (ETU). The interaction of physics/engineering on the development side, with researchers from natural sciences, medicine and material sciences on the application side, and the well-founded training strengthens Erlangen as a research location and has established OICE nationally and also internationally as a recognised institute for light microscopy. The novel combination of real-time super resolution with spinning disc laser scanning allows for the first time long-term measurements of living three-dimensional samples with improved lateral resolution and, in combination with improved silicone immersion objectives, higher penetration depths with lower artefacts of > 100 µm. However, the speed and significantly lower illumination intensity of an SD-LSM is retained. Thus, this Super Resolution SD-LSM for long-term live cell observation replaces the previous SD-LSM (acquired in 2013) and complements the existing devices such as the multiphoton (intravital imaging) LSM and light sheet microscopy (fixed samples). At the OICE, there is still a high demand for spinning disc technology, especially considering the technological advancements in resolution, penetration depth and better camera detectors. The majority of the > 30 research groups/year that successfully use this technology use organoid models, live cell cultures or tissues to perform long-term spatio-temporal multichannel imaging (from several hours to whole days). The highest possible lateral (super) resolution for dynamic intracellular or membrane-associated processes is essential. The high thickness of the samples (organoids / tissue) requires a high penetration depth with minimised scattering to enable meaningful three-dimensional reconstruction and measurements, also over time. A large optical field of view that is as homogeneously illuminated as possible also minimises tile imaging with downstream stitching, which is associated with losses. In the medium term, this device should also relieve and replace the classic SD-LSM, which has now been in continuous operation for ten years (> 2,600 hours/year).

OICE是FAU的卓越中心（中央研究所），代表了使用基于光基技术的高高和超高分辨率成像的集中式平台，除其他外，生物细胞和组织，器官，弹性成像以及生物学材料和机制的成像。 OICE结合了核心设施单元（CFU），探索性研究部门（ERU）和教育培训部（ETU）。物理/工程在开发方面的相互作用与应用方面的自然科学，医学和物质科学的研究人员以及有充分根据的培训优势Erlangen作为研究地点，并在全国范围内建立了OICE，并在国际上建立了公认的光学显微镜研究所。实时超级分辨率与旋转盘激光扫描的新型组合允许首次长期测量生存的三维样品，具有改善的侧向分辨率，并结合改善的硅胶浸入目标，更高的渗透深度，较低的人工伪像> 100 µm。但是，保留了SD-LSM的速度和显着降低的照明强度。这是长期活细胞观察的这种超级分辨率SD-LSM取代了先前的SD-LSM（2013年获得），并符合现有的设备，例如多光（插入式成像）LSM和光片显微镜（固定样品）。在OICE，仍然对旋转光盘技术的需求仍然很高，尤其是考虑到分辨率，穿透深度和更好的相机探测器的技术进步。成功使用该技术的30个研究小组中的大多数研究小组使用器官模型，活细胞培养或时间来执行长期时空多通道成像（从几个小时到整天）。动态内或膜相关过程的最高横向（超级）分辨率是必不可少的。样品的高厚度（类器官 /组织）需要高渗透深度，并且散射最小，以实现有意义的三维重建和测量，也随着时间的流逝。尽可能均匀地照亮的大型光场，也通过下游缝线进行最小的瓷砖成像，这与损失有关。在中期，该设备还应挽救和替换经典的SD-LSM，该SD-LSM现在连续运行已有十年（> 2,600小时/年）。