Spinning Disk Super Resolution Confocal Microscope for the Vanderbilt Brain Institute

范德比尔特脑研究所的转盘超分辨率共焦显微镜

基本信息

批准号：
10721222
负责人：
Jenny Schafer
金额：
$ 59.78万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10721222
关键词：
3-Dimensional Academic Medical Centers Aggressive behavior Area Attention deficit hyperactivity disorder Award Basic Science Brain Cells Communication Computers Core Facility Dedications Equipment Faculty Fees Financial Support Funding Image Impulsivity Incubated Institution Interdisciplinary Study Investigation Lasers Learning Medical Research Memory Mental Health Microscope Microscopy Minor Molecular National Institute of Mental Health Neurosciences Organoids Population Recording of previous events Research Research Institute Research Personnel Resolution Resource Sharing Rett Syndrome Sampling Signal Transduction Social Interaction Specialist Speed Synapses System Tissues Training United States National Institutes of Health Universities Wages cellular imaging confocal imaging instrument lens live cell imaging microscopic imaging new technology operation superresolution imaging synaptogenesis ultra high resolution

项目摘要

PROJECT SUMMARY / ABSTRACT Funds are requested to purchase a Nikon Spinning Disk Super Resolution (CSU-W1/SoRa) Confocal microscope for imaging projects focused around mental health research within the Vanderbilt Brain Institute (VBI), a multi-disciplinary research institute comprised of faculty at Vanderbilt University (VU) and Vanderbilt University Medical Center (VUMC). The requested instrument will be integrated into and managed by the Vanderbilt Cell Imaging Shared Resource (CISR), a microscopy core with a 20+ year history of successful microscopy management, training, and acquisition of new technology. This instrument capable of confocal imaging and super resolution imaging in live samples is ideal for 6 Major and 7 Minor Users within the VBI with projects surrounding synaptogenesis, synapse assembly and plasticity, subsynaptic signaling, and communication between cell and tissue populations. These molecular and cellular investigations will advance already funded NIMH and NIH projects totaling over $6.8M in FY22 in the areas of social interaction, impulsive aggression, learning and memory, Rett syndrome (RTT), ADHD, and more. The proposed microscope configuration is based on the needs of the VBI and Major Users for live imaging at cellular and subcellular resolution. The Nikon CSU-W1 SoRa confocal microscope includes: 1) CSU-W1 spinning disk plus Yokogawa SoRa super resolution spinning disk for super resolution near 120 nm in XY; 2) stage-top incubation for imaging live cells and organoids; 3) Z-Piezo stage for increased speed in Z for 3D volumes; 4) Hamamatsu Orca-Fusion sCMOS camera with 30-100 frames per second; 5) excitation lasers of 405, 488, 561, and 633 nm; and 6) objective lenses for live cell imaging at high resolution ranging from 10x, 20x, 40x, 60x, and 100x. This new system would be housed in existing CISR space in Medical Research Building III (MRBIII) where the VBI is based and the majority of the Major User labs are located. Vanderbilt University is providing financial support to promote the long-term operation of the instrument. If awarded, the Office of the Dean of Basic Sciences is committed to providing $75,000 for CISR staff salary, computer purchases and upgrades, and reduction in user fees over the first 5 years. Technical support and training of new users will be facilitated by the exceptional staff within the CISR, which has a greater than 20-year history of assisting over 300 labs on campus. Moreover, this equipment will become part of the Vanderbilt Nikon Center of Excellence (COE) that is housed within the CISR. This CISR/Nikon COE provides a dedicated local team of specialists to support the microscopy needs of investigators on campus. Collectively, the proposed confocal system, together with outstanding institutional and core facility support, will enable the long-term objective of supporting and advancing neuroscience and mental health research Vanderbilt.

项目摘要 /摘要要求资金购买尼康旋转磁盘超级分辨率（CSU-W1/SORA）共焦成像项目的显微镜围绕范德比尔特脑研究所内的心理健康研究（VBI），由范德比尔特大学（VU）和范德比尔特教职员工组成的多学科研究所大学医学中心（VUMC）。所请求的工具将由 Vanderbilt Cell Imaging共享资源（CISR），这是一个超过20年历史的显微镜核心新技术的显微镜管理，培训和获取。这个能够共焦的仪器现场样品中的成像和超级分辨率成像是VBI中6个主要和7个次要用户的理想选择围绕突触发生，突触组件和可塑性，突触次触及信号传导和的项目细胞和组织种群之间的通信。这些分子和细胞研究将进展已经为NIMH和NIH项目提供了资金，在社会互动领域，总计超过680万财年侵略，学习和记忆，RETT综合征（RTT），ADHD等。所提出的显微镜配置基于VBI的需求和主要用户对实时成像的需求细胞和亚细胞分辨率。尼康CSU-W1 Sora共聚焦显微镜包括：1）CSU-W1 旋转磁盘加上Yokogawa Sora超级分辨率旋转磁盘，可在XY中120 nm附近的超级分辨率； 2）用于成像活细胞和器官的阶段孵育； 3）Z-Piezo阶段，用于增加Z的速度3D 卷； 4）Hamamatsu-Fusion SCMOS摄像头，每秒30-100帧； 5）激发激光器 405、488、561和633 nm; 6）高分辨率的活细胞成像的客观镜头，范围为10倍，20倍 40倍，60倍和100倍。这个新系统将安置在医学研究大楼的现有CISR空间中（MRBIII）在VBI所基于的并且大多数主要用户实验室所在的位置。范德比尔特大学（Vanderbilt University）正在提供财政支持，以促进该工具的长期运作。如果授予基础科学院长办公室，致力于为CISR员工工资提供75,000美元计算机购买和升级，以及最初5年的用户费用减少。技术支持和 CISR中的杰出人员将促进对新用户的培训，该员工的培训超过20年在校园协助300多个实验室的历史。此外，该设备将成为范德比尔特尼康的一部分卓越中心（COE）位于CISR中。这个CISR/Nikon Coe提供了专用的本地专家团队支持校园调查人员的显微镜需求。共同提议共聚焦系统以及出色的机构和核心设施的支持，将使能够长期支持和推进神经科学和心理健康研究范德比尔特的目标。