Novel nanoparticle-antibody conjugates for biotechnology applications

用于生物技术应用的新型纳米颗粒-抗体缀合物

• 批准号: 488712-2015
• 负责人: Algar, Russ
• 金额: $ 1.82万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=586375
• 关键词: Novel; nanoparticle; antibody; conjugates; biotechnology

The convergence of nanotechnology and biotechnology can lead to new and improved methods for biomedical research and development, which will ultimately improve health care in Canada and worldwide. For example, nanoparticles composed of gold, semiconductors, and magnetic materials such as iron oxide have unique properties that can benefit diagnostic tests for molecular markers of disease, or the isolation of specific cell types for study. Vancouver-based STEMCELL Technologies Inc. has a portfolio of iron oxide nanoparticle and antibody products that can be used for these applications. These products have been enabled by iron oxide nanoparticles and a patented "tetrameric antibody complex (TAC)" method of linking the nanoparticles with antibodies. Many other types of nanoparticles are useful for a range of other biological applications, but have not be utilized with the TAC method. We propose to collaborate with STEMCELL Technologies to adapt their TAC methodology to gold and semiconductor nanoparticles using simple, modular, and reliable chemistry. The antibody-nanoparticle conjugates will then be physically characterized and validated in biochemical assays. This project will yield new methods from preparing biologically functional nanoparticles, support the training of highly qualified personnel with skills valued by local scientific industry, enhance the technology and (potentially) product portfolio of STEM CELL Technologies, and lead to future collaboration on the development of novel biomedical methods and technologies.

纳米技术和生物技术的融合可以带来新的和改进的生物医学方法 研究和开发，最终将改善加拿大和全世界的医疗保健。例如， 由金、半导体和氧化铁等磁性材料组成的纳米粒子具有独特的 有助于疾病分子标记物诊断测试或特定细胞分离的特性 供研究的类型。总部位于温哥华的 STEMCELL Technologies Inc. 拥有氧化铁纳米颗粒和 可用于这些应用的抗体产品。这些产品是通过氧化铁实现的 纳米颗粒和获得专利的“四聚体抗体复合物（TAC）”方法将纳米颗粒与 抗体。许多其他类型的纳米颗粒可用于一系列其他生物应用，但 不能与 TAC 方法一起使用。我们建议与 STEMCELL Technologies 合作，以适应他们的 使用简单、模块化且可靠的化学方法对金和半导体纳米颗粒进行 TAC 方法。这 然后，抗体-纳米颗粒缀合物将在生化测定中进行物理表征和验证。 该项目将产生制备生物功能纳米粒子的新方法，支持培训 培养具有当地科学产业重视的技能的高素质人才，提高技术和水平 （潜在）STEM CELL Technologies 的产品组合，并促成未来在 开发新的生物医学方法和技术。