Development and manufacture of self-assembled, multifunctional composite nanoparticles with magnetic and fluorescent properties for cell-based assays

开发和制造具有磁性和荧光特性的自组装多功能复合纳米粒子，用于基于细胞的分析

• 批准号: 493833-2016
• 负责人: Algar, Russ
• 金额: $ 12.35万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=643732
• 关键词: Development; manufacture; self; assembled; multifunctional

Canada's health care system is overburdened with both cost and demand, but at the same time critically needs new technologies that can deliver advances in diagnostics and personalized medicine to Canadians. These new technologies must therefore be cost-effective and easily deployed. The manufacture of composite NPs can enable such technologies. We propose to develop and manufacture multifunctional composite nanoparticles that combine magnetic properties with ultra-bright fluorescence. These particles will combine several component materials, resulting in new composite nanoparticles with capabilities that exceed those of the component materials individually. The component materials include fluorescent semiconductor quantum dots (QDs), semiconducting polymer dots (Pdots), and amphiphilic chemically-functionalized polymers from our laboratories, as well as magnetic iron oxide nanoparticles from our industry partner, Vancouver-based STEMCELL Technologies Inc. The polymer and QD or PDot components will be self-assembled in a layered, hierarchical structure around the central magnetic nanoparticle, then further self-assembled with an outer layer of bioactive molecules such as tetrameric antibody complexes (also from STEMCELL) or nucleic acid aptamers that we will develop. The self-assembly processes will provide the control and reproducibility necessary for the automated manufacture, scale-up, and online characterization of the composite nanoparticles, which is a process that we also propose to develop. The combined brightness, magnetism, and bioactivity of the composite nanoparticle will enable the simple, low-cost separation of target cells from complex samples (e.g., blood) with detection and counting of the target cells on a smartphone. A peripheral device will be developed for the smartphone to enable this capability. The proposed technology is potentially transformative in that it can make cell-based diagnostic assays amenable to point-of-care diagnostics and personalized medicine (e.g., detection of circulating tumour cells), surpassing current technologies that are costly, require special training, and are only available in specialized labs.**************

加拿大的医疗保健系统对成本和需求既负担重大，但同时批判性需要新技术，这些技术可以为加拿大人提供诊断和个性化医学的进步。因此，这些新技术必须具有成本效益且容易部署。复合NP的制造可以实现此类技术。我们建议开发和制造将磁性特性与超亮荧光相结合的多功能复合纳米颗粒。这些颗粒将结合几种组件材料，从而导致新的复合纳米颗粒具有分别超过组件材料的功能。组件材料包括荧光半导体量子点（QD），半导体聚合物点（PDOT）和来自我们实验室的两亲性化学功能化聚合物，以及来自我们行业的氧化铁纳米粒子，来自我们行业的氧化铁纳米粒子。 QD或PDOT成分将在中央磁性纳米颗粒周围的分层，分层结构中进行自组装，然后再用生物活性分子的外层进行自组装，例如四聚体抗体复合物（也来自stemcell）或核酸适中，我们会发展。自组装过程将为复合纳米颗粒的自动制造，扩展和在线表征提供所需的控制和可重复性，这是我们也建议开发的过程。复合纳米颗粒的亮度，磁性和生物活性将使目标细胞与复杂样品（例如血液）的简单，低成本分离，并在智能手机上检测和计数目标细胞。将为智能手机开发外围设备，以启用此功能。该提出的技术具有潜在的变革性，因为它可以使基于细胞的诊断测定法适用于护理点诊断和个性化医学（例如，检测到循环肿瘤细胞），超过当前的技术，需要经过特殊培训，并且是经过特殊培训，并且是经过特殊培训，并且是仅在专业实验室中可用。**************