Copper Acquisition by Methanotrophs.

甲烷氧化菌对铜的获取。

基本信息

批准号：
9036409
负责人：
Laura Dassama
金额：
$ 5.8万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-04-01 至 2017-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9036409
关键词：
Active Biological Transport Affinity Alkanesulfonates Anabolism Animal Model Bacteria Binding Biochemical Bioinformatics Biological Process Biosynthetic Proteins Carbon Carbon Dioxide Carrier Proteins Cells Complex Copper Cysteine Disulfides Effectiveness Environment Enzymes Escherichia coli Exhibits Gases Goals Gram-Negative Bacteria Health Homeostasis Human Activities Integral Membrane Protein Investigation Kinetics Lead Literature Membrane Metals Methane Methane Metabolism Pathway Methane hydroxylase Methanol Methylocystis Methylosinus trichosporium Natural Products Nutrient Operon Oxazolone Particulate Phenotype Physiological Post-Translational Protein Processing Proteins Radiation Reaction Research Role Route Source Structure Substrate Specificity Thioamides Threonine Transmembrane Domain United States Vertebral column Work base biophysical techniques cofactor greenhouse gases insight oxidation receptor remediation small molecule

项目摘要

DESCRIPTION (provided by applicant): Methane is the second most prevalent greenhouse gas emitted in the United States as a result of human activity. Because of methane's effectiveness at trapping radiation, it is considered significantly more deleterious to the environment than carbon dioxide. Methanotrophs are gram-negative bacteria that metabolize methane and may provide routes for the remediation of the gas. In the first step of metabolizing methane, methanotrophs use an integral membrane protein called particulate methane monooxygenase (pMMO) to oxidize methane to methanol. pMMO is copper-dependent enzyme, and thus methanotrophs have an elevated requirement for copper (Cu). Under conditions of limiting Cu, some methanotrophs secrete a small natural product-like molecule that binds Cu with high affinity. This molecule, called methanobactin (Mbn), is re- internalized into the cell in its Cu-loaded form to satisfy the cell's copper needs. The proposed research will characterize the transporter implicated in the re-internalization of the Cu-Mbn complex in two different methanotrophs. This work will provide insight into the mechanism by which a key requirement for methane metabolism, the acquisition of Cu for pMMO's cofactor, is fulfilled. Additionally, it will provide insight on methanotrophic bacterial homeostasis.

描述（由申请人提供）：甲烷是美国人类活动排放的第二大温室气体。由于甲烷能够有效捕获辐射，因此人们认为它对环境的危害比二氧化碳大得多。甲烷氧化菌是代谢甲烷的革兰氏阴性细菌，可能为气体的修复提供途径。在代谢甲烷的第一步中，甲烷氧化菌使用一种称为颗粒甲烷单加氧酶（pMMO）的完整膜蛋白将甲烷氧化为甲醇。 pMMO 是铜依赖性酶，因此甲烷氧化菌对铜 (Cu) 的需求量较高。在限制铜的条件下，一些甲烷氧化菌会分泌一种类似天然产物的小分子，以高亲和力结合铜。这种分子称为甲烷菌素 (Mbn)，在体内重新内化到细胞中。其负载铜的形式可以满足电池对铜的需求。拟议的研究将表征两种不同甲烷氧化菌中与 Cu-Mbn 复合物重新内化有关的转运蛋白。这项工作将深入了解满足甲烷代谢的关键要求（pMMO 辅因子 Cu 的获取）的机制。此外，它将提供有关甲烷氧化细菌稳态的见解。