Structural Biology Shared Service

结构生物学共享服务

基本信息

批准号：
9145400
负责人：
David Joseph Weber
金额：
$ 12.55万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-04 至 2021-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9145400
关键词：
Address Americas Award Baltimore Biological Biometry Cancer Center Cancer Center Support Grant Cigarette Computer Assisted Consultations County Crystallization Data Data Collection Databases Deposition Development Doctor of Philosophy Drug Design Enzymes Flow Cytometry Funding Genomics Grant Head Hormone Responsive Human Image Institutes Institution Investigational Therapies Journals Laboratories Malignant Neoplasms Maryland Medical Medical center Methodology Methods Mission Molecular Molecular Biology Molecular Mechanisms of Action Molecular Probes Nature Nuclear Magnetic Resonance Pathology Peer Review Pharmaceutical Chemistry Pharmacy Schools Population Sciences Preparation Protein Databases Proteins Public Health Schools Publications Publishing Reader Research Research Personnel Resolution Resources Robotics Roentgen Rays Sampling School Dentistry Services Structure System Therapeutic Training Translational Research United States United States National Academy of Sciences United States National Institutes of Health Universities X ray diffraction analysis X-Ray Crystallography X-Ray Diffraction abstracting acronyms biobank collaborative environment college computerized data processing design drug candidate high throughput screening instrument instrumentation investigator training medical schools member neoplasm immunotherapy novel pre-clinical programs small molecule inhibitor small molecule libraries structural biology training opportunity tumor immunology virology

项目摘要

6.0 Abstract: Structural Biology Shared Service The Structural Biology Shared Service (SBSS) provides investigators with the facilities and expertise to collect and interpret molecular structural information (nuclear magnetic resonance [NMR], X-ray crystallography) to understand the molecular mechanism(s) of action of proteins and enzymes that contribute to cancer. The SBSS also facilitates identification of molecular agents (e.g., candidate drugs, molecular probes) that can be used to study the function of cancer targets and may have therapeutic potential. To achieve this mission, the SBSS implements a structural biology approach. This includes isolation and characterization of samples for NMR and X ray and application of state-of-the-art data collection methods in a collaborative environment that facilitates access by UMGCC investigators. In 2014, 36 UMGCC investigators used the SBSS. These investigators deposited 84 high-resolution structures into the protein database in addition to publishing in high- impact journals. The efforts of the SBSS have facilitated development of several novel, cancer target–specific small-molecule inhibitors. Interactions between the SBSS and investigators from multiple UMGCC programs have facilitated the integration of structural biology into the translational research efforts of UMGCC. Also of significance, the SBSS installed and commissioned a new 950 MHz NMR spectrometer purchased through an $8 million NIH Shared Instrumentation Grant. This is the only such instrument available in an academic institution in the United States and represents the largest Shared Instrumentation Grant awarded by NIH. The SBSS has also expanded and upgraded X-ray diffraction and robotic crystallization resources to enhance the use of these resources. In 2014, the SBSS supported 36 Cancer Center members spanning all 5 research programs (14 percent of all UMGCC members); 53 percent of UMGCC users have peer-reviewed funding. The SBSS supports many cancer-related publications annually, many in high-impact journals including Proceedings of the National Academy of Sciences of the United States of America, Journal of Medicinal Chemistry, and Nature Structural & Molecular Biology.

6.0 摘要：结构生物学共享服务结构生物学共享服务 (SBSS) 为研究人员提供收集数据的设施和专业知识并解释分子结构信息（核磁共振 [NMR]、X 射线晶体学）了解导致癌症的蛋白质和酶的分子作用机制。 SBSS 还有助于分子制剂（例如候选药物、分子探针）的鉴定，这些分子制剂可以用于研究癌症靶点的功能并可能具有治疗潜力。 SBSS 采用结构生物学方法，其中包括样品的分离和表征。 NMR 和 X 射线以及在协作环境中应用最先进的数据收集方法，方便 UMGCC 调查人员进行访问 2014 年，36 名 UMGCC 调查人员使用了 SBSS。研究人员除了以高分辨率发表之外，还将 84 个高分辨率结构存入蛋白质数据库。 SBSS 的努力促进了多本针对特定癌症目标的小说的开发。 SBSS 与多个 UMGCC 项目的研究人员之间的相互作用。促进了结构生物学与 UMGCC 转化研究工作的整合。意义重大的是，SBSS 安装并调试了一台通过一家公司购买的新型 950 MHz NMR 波谱仪。 800 万美元的 NIH 共享仪器拨款这是学术界唯一可用的此类仪器。美国国立卫生研究院 (NIH) 授予的最大的共享仪器拨款。 SBSS还扩展和升级了X射线衍射和机器人结晶资源，以增强使用这些资源。 2014 年，SBSS 支持了涵盖所有 5 个研究项目的 36 名癌症中心成员（占所有研究项目的 14%） 53% 的 UMGCC 用户拥有经过同行评审的资金 SBSS 支持许多项目。每年发表与癌症相关的出版物，其中许多发表在高影响力期刊上，包括Proceedings of the National 美国科学院、Journal of Medicinal Chemistry 和 Nature Structural & 分子生物学。