Analytical Core: Extraction, Detection, and Interpretation of PCB Congeners in Cmplex Matrices

分析核心：复杂矩阵中 PCB 同系物的提取、检测和解释

• 批准号: 9149263
• 负责人: Keri C Hornbuckle
• 金额: $ 25.74万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9149263
• 关键词: Address; Adult; Agreement; Air; Animals; Archives; Area; Basic Science; Biological; Body Burden; Breathing; Calibration; Characteristics; Chemicals; Child; Complex; Databases; Detection; Development; Electronics; Environment; Environmental Pollutants; Environmental sludge; Foundations; Frequencies; Furniture; Goals; Health; Human; Human Resources; Hydroxyl Radical; Individual; Injection of therapeutic agent; Inorganic Sulfates; Institutes; Iowa; Journals; Laboratories; Laboratory Animals; Link; Maintenance; Measurement; Measures; Metabolic; Metabolism; Methods; Molecular Weight; Mus; Paint; Parents; Peer Review; Plant Resins; Plants; Policies; Polychlorinated Biphenyls; Procedures; Process; Program Research Project Grants; Property; Protocols documentation; Publishing; Quality Control; Quartz; Rattus; Recommendation; Recording of previous events; Reference Standards; Reproducibility; Research; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resistance; Sampling; Schools; Serum; Services; Shipping; Ships; Signal Transduction; Soil; Source; Sunlight; Superfund; Technology; Time; Tissues; Toxic effect; Training; United States Environmental Protection Agency; Universities; Unspecified or Sulfate Ion Sulfates; Urine; air sampling; animal tissue; building materials; community based participatory research; data sharing; design; high throughput analysis; innovation; instrument; instrumentation; manufacturing process; meetings; method development; photolysis; pollutant; polyurethane foam; programs; quality assurance; repaired; response; signal processing; tandem mass spectrometry; water sampling

PROJECT SUMMARY PCB analysis for research purposes is labor-intensive, requires specialized instrumentation, and depends on excellent quality control procedures. Because the Analytical Core provides the trained staff, maintains the instruments and facilities, and provides methods to assure accuracy, precision, representativeness, comparability, and reproducibility, the isrp research projects are able to make high impact discoveries, publish their results in peer-review journals, and more quickly provide scientific guidance regarding PCBs in the environment and their effect on human health. The Analytical Core supports the research of the isrp by extracting and analyzing samples for all 209 PCBs and for individual compounds in complex matrices including air samples; harbor sediment; human blood serum; urine; laboratory animal tissues; plant tissues; soils; sludge; laboratory solutions, building materials, paint, and furniture. Each year the Analytical Core will complete thousands of such measurements as well as hundreds of quality control measurements and method development measurements. The Analytical Core provides detailed quality control guidance and collaborates closely with isrp researchers to determine appropriate methods for quality assurance control. Analyses for PCB congeners in environmental matrices includes routine use of surrogate standards injected in every sample to assess and correct for analytical efficiency; internal standards for quantification; duplicate injections; laboratory blanks, calibration standards, and frequent analysis of materials with certified concentrations of PCBs. The rigor of the QC/QA protocol is unusual for PCB analysis but provides the critical foundation for discoveries in the research projects. The Analytical Core has a sustained history of innovation, starting with the development of tandem mass spectrometry for congener-specific analysis of PCBs in environmental and laboratory samples. This method enabled many important discoveries and innovative findings in the isrp, including the ability to link specific toxic properties by compound; accurate determination of the lower molecular weight compounds; and precise measurement of all PCBs. The congener-specific method used by the Analytical Core enabled the discovery of non-Aroclors in the environment. The study of these compounds, which are currently produced through some chemical manufacturing processes, has been a major area of focus since that time. Innovations planned include the development of efficient methods for routine analysis of known and as-yet unknown decay products of PCBs. Although PCBs are very persistent and resistant to decay, many PCBs can be broken down by sunlight and by metabolism in plants, animals and humans. The Analytical Core assists the research projects in measuring those decay products. .

项目概要 用于研究目的的 PCB 分析是劳动密集型的，需要专门的仪器，并且取决于 优秀的质量控制程序。因为分析核心提供了经过培训的人员，维持了 仪器和设施，并提供确保准确性、精密度、代表性的方法， 具有可比性和可重复性，isrp 研究项目能够做出具有重大影响的发现、发表 他们的结果发表在同行评审期刊上，并更快地提供有关 PCB 的科学指导 环境及其对人类健康的影响。 分析核心通过提取和分析所有 209 个 PCB 的样本来支持 isrp 的研究 以及复杂基质（包括空气样品）中的单个化合物；港口沉积物；人血 血清;尿;实验动物组织；植物组织；土壤；污泥;实验室解决方案、建筑材料、 油漆、家具。每年，分析核心都会完成数千次此类测量以及 数百种质量控制测量和方法开发测量。分析核心 提供详细的质量控制指导，并与 ISRP 研究人员密切合作，以确定 适当的质量保证控制方法。环境基质中 PCB 同系物的分析 包括常规使用注入每个样品的替代标准品来评估和校正分析 效率;量化的内部标准；重复注射；实验室空白、校准标准、 经常分析含有经过认证的多氯联苯浓度的材料。 QC/QA 协议的严格性是 对于 PCB 分析来说不寻常，但却为研究项目的发现提供了重要的基础。 分析核心拥有持续的创新历史，始于串联质量的开发 用于环境和实验室样品中 PCB 同类物特异性分析的光谱测定法。这个方法 在 ISRP 中实现了许多重要的发现和创新发现，包括链接特定有毒物质的能力 化合物的特性；准确测定低分子量化合物；和精确的 所有 PCB 的测量。分析核心使用的同类物特定方法能够发现 环境中不含 Aroclors。目前通过一些方法生产这些化合物的研究 自那时以来，化学制造工艺一直是人们关注的主要领域。计划创新 包括开发对已知和未知的衰变产物进行常规分析的有效方法 PCB 的数量。尽管多氯联苯非常持久且耐腐烂，但许多多氯联苯可以通过以下方法分解： 阳光以及植物、动物和人类的新陈代谢。分析核心协助研究项目 在测量那些衰变产物时。 。