Develop a Dual Functional Nanoprobe for Integration of Fluorescence microscopy wi

开发用于集成荧光显微镜的双功能纳米探针

基本信息

批准号：
8047401
负责人：
Jie Zheng
金额：
$ 21.04万
依托单位：
UNIVERSITY OF TEXAS DALLAS
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8047401
关键词：
Address Affinity Binding Binding Proteins Biology Cell physiology Cells Chemical Structure Chemicals Complex Electron Microscopy Environment Event Fluorescence Fluorescence Microscopy Fluorescence Resonance Energy Transfer Fluorescent Probes Folic Acid Foundations Goals Homocysteine Homocystine Image Imaging Device Imaging Techniques In Situ In Vitro Label Life Ligands Location Measures Medicine Microscopic Microscopy Molecular Nanotechnology Nucleotide Biosynthesis Play Proteins Raman Spectrum Analysis Reaction Reporting Research Resolution Role Signal Transduction Silver Spectrum Analysis Structure Surface Techniques Time Work X-Ray Crystallography base bioimaging chemical reaction disease diagnosis folate-binding protein imaging probe in vivo interest nanoparticle nanoprobe nanoscience novel therapeutics particle programs protein complex receptor receptor binding response solid state success therapeutic development therapy development

项目摘要

DESCRIPTION (provided by applicant): The objective of the proposed application is to develop a dual functional probe which is detectable at single-particle level for both Raman and fluorescence microscopy, so that the strengths of each technique can be combined into a single imaging tool for tackling challenges in bioimaging. With this new imaging probe and tool, we plan to address a long-term bioimaging challenge that is real-time imaging of interactions between ligands and receptors at the molecular level inside live cells. Success of this proposed work will (1) provide a new imaging probe that is not only highly fluorescent and robust but can also report molecular information for ligands it labels; (2) offer a new imaging tool which integrates strengths of fluorescence microscopy and Raman spectroscopy; (3) enable us to correlate cellular dynamics of ligands with ligand-binding protein interactions at the chemical level inside live cells. The applications of these fluorescent and Raman probes are not limited to the proposed studies; they can also find applications in bioimaging as multiplexing and multifunctional probes to correlate fluorescence and Raman images with electron microscopy images at in vitro and in vivo level. PUBLIC HEALTH RELEVANCE: Ligand-receptor interactions play a pivotal role in regulating cellular functions and provide a foundation for disease diagnosis and new therapy development. However, comprehensive understanding of in vivo interactions between ligand and receptors at the molecular level remains highly challenging. Our research application aims to integrate fluorescence microscopy with Raman spectroscopy using a dual functional probe, so that we can simultaneously track and chemically image of ligand-receptor complexes at the single-cell level inside live cells. Success of this proposed work will (1) provide a new imaging probe that not only is highly fluorescent and robust but also can report molecular information of ligands it labels; (2) offer a new imaging tool which integrate strengths of fluorescence microscopy and Raman spectroscopy; (3) enable us to correlate cellular dynamics of ligands with ligand-receptor interactions at the chemical level inside live cells.

描述（由申请人提供）：所提出申请的目的是开发一种双功能探针，可在拉曼和荧光显微镜的单颗粒水平上进行检测，以便将每种技术的优点结合到一个成像工具中应对生物成像领域的挑战。借助这种新的成像探针和工具，我们计划解决长期生物成像挑战，即在活细胞内分子水平上对配体和受体之间的相互作用进行实时成像。这项工作的成功将（1）提供一种新的成像探针，它不仅具有高荧光性和鲁棒性，而且还可以报告其标记的配体的分子信息；（2）提供一种新的成像工具，融合了荧光显微镜和拉曼光谱的优点；（3）使我们能够将配体的细胞动力学与活细胞内化学水平上的配体结合蛋白相互作用相关联。这些荧光和拉曼探针的应用并不限于所提出的研究；他们还可以在生物成像中作为多重和多功能探针来将荧光和拉曼图像与体外和体内水平的电子显微镜图像相关联。公共卫生相关性：配体-受体相互作用在调节细胞功能中发挥着关键作用，并为疾病诊断和新疗法开发提供基础。然而，在分子水平上全面了解配体和受体之间的体内相互作用仍然极具挑战性。我们的研究应用旨在使用双功能探针将荧光显微镜与拉曼光谱相结合，以便我们可以在活细胞内的单细胞水平上同时跟踪配体-受体复合物并进行化学成像。这项工作的成功将（1）提供一种新的成像探针，它不仅具有高荧光性和鲁棒性，而且可以报告其标记的配体的分子信息；（2）提供一种新的成像工具，融合了荧光显微镜和拉曼光谱的优点； (3) 使我们能够将配体的细胞动力学与活细胞内化学水平上的配体-受体相互作用相关联。