SYNTHESIS AND MASS SPECTROMETRY ANALYSIS OF NUCLEOSIDE ANALOGS

核苷类似物的合成和质谱分析

• 批准号: 8168767
• 负责人: LARRY W MCLAUGHLIN
• 金额: $ 0.01万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-10 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168767
• 关键词: Antiviral Agents; Area; Characteristics; Complementary DNA; Computer Retrieval of Information on Scientific Projects Database; Crystallization; DNA; DNA Sequence; Exhibits; Funding; Gene Expression; Goals; Grant; Human; Institution; Mass Spectrum Analysis; Pharmacologic Substance; Polymerase; Process; Research; Research Personnel; Resources; Site; Source; Structure; System; United States National Institutes of Health; Virus; Wit; controlled release; nanoscale; nucleobase; nucleoside analog; scaffold; solute; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The McLaughlin group's research spans three related areas: (1) they prepare nucleoside analogues as potential antivirals. They examine structural alterations on both the nucleobase as well as the sugar. Their goal is to develop compounds that exhibit differential activity with polymerase - active with the virus, inactive wit human polymerases - this will help to develop new anti-virals. (2) Understanding macromolecular recognition processes is critical to understanding gene expression. To probe such processes, they prepare modified DNA sequences in which the recognition sites are functionally altered. They use these altered DNA sequences to probe structure, function and recognition. (3) The natural structural characteristics of DNA lend themselves to the construction of repeating lattices formed by hybridization of complementary DNA sequences. These nanoscale structures can be used as crystallization scaffolds for multi-component systems, to sequester nanometer sized solutes or for controlled release of nanoscale pharmaceuticals.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 麦克劳克林小组的研究跨越三个相关领域：（1）他们制备核苷类似物作为潜在的抗病毒药物。他们检查核碱基和糖的结构变化。他们的目标是开发对聚合酶表现出不同活性的化合物——对病毒有活性，对人类聚合酶无活性——这将有助于开发新的抗病毒药物。 (2)了解大分子识别过程对于了解基因表达至关重要。为了探究这些过程，他们制备了修饰的 DNA 序列，其中识别位点在功能上发生了改变。他们利用这些改变的 DNA 序列来探测结构、功能和识别。 (3) DNA 的天然结构特征有助于构建由互补 DNA 序列杂交形成的重复晶格。这些纳米级结构可用作多组分系统的结晶支架，以隔离纳米级溶质或用于纳米级药物的受控释放。