WFUSM Macaca fascicularis SPF Breeding Colony

WFUSM 食蟹猴 SPF 育种群

基本信息

批准号：
7798161
负责人：
JANICE D. WAGNER
金额：
$ 120.02万
依托单位：
WAKE FOREST UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-01 至 2012-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7798161
关键词：
Age Animal Feed Animal Sources Animals Area Binding Biological Biomedical Research Breeding Cardiovascular Diseases Cardiovascular system Cercopithecine Herpesvirus 1 Chronic Disease Cognition Collaborations Communicable Diseases Coronary heart disease Custom DNA analysis Data Dependence Development Diabetes Mellitus Diet Diet Research Disease Disease model Earthquakes Endocrine Disruptors Estrogen Receptors Excision Exposure to Extramural Activities Floods Funding Genetic Health Heritability Herpesviridae Hurricane Immune System Diseases Immune response Immunology Individual International Interruption Investigation Isoflavones Macaca Macaca fascicularis Macronutrients Nutrition Malignant Neoplasms Metabolic Modeling Monitor Monkeys Mycobacterium tuberculosis Natural Disasters Nutritional Osteoporosis Outcome Performance Personal Communication Pesticides Phenotype Phytoestrogens Postmenopause Primates Quarantine Research Research Infrastructure Research Personnel Research Project Grants Resources Risk Risk Factors Rodent SIV Scientist Seeds Shipping Ships Simian Retroviruses Source Specific Pathogen Frees Terrorism Tornadoes Tuberculosis United States National Institutes of Health Universities Variant Virus Washington Women&apos s Health Work animal colony design disorder risk experience father role feeding fetal programming forest germ free condition interest male malignant breast neoplasm medical schools nonhuman primate offspring pathogen programs reproductive simian virus skeletal soy soy protein isolate transmission process virology

项目摘要

DESCRIPTION (provided by applicant): It is generally recognized that nonhuman primates comprise an invaluable resource for biomedical research. The cynomolgus monkey (Macaca fascicularis) is one of the two most widely used macaque species, with biomedical applications in infectious disease and immune disorders, diabetes and cardiovascular disease, women's health, osteoporosis, and reproductive tract cancer. We proposed to expand an existing demonstration colony of specific pathogen free (SPF) cynomolgus monkeys to function as a national resource for NIH grantees. This proposal involves a collaboration between Wake Forest University School of Medicine (WFUSM) and the Washington National Primate Research Center (WaNPRC). WFUSM will provide infrastructure, colony management, genetic monitoring, and expertise in chronic disease modeling (cardiovascular, skeletal, metabolic, and oncologic disorders), while the WaNPRC contributes expertise in virology, immunology, and SPF breeding. Establishment of a domestic breeding colony of defined pathogen status will provide many advantages, including direct access to source animals and insulation from potential interruptions of supply due to political unrest, terrorism, shipping delays, and international quarantine. The colony will be located in a region at low risk of natural disasters (flood, hurricane, tornado, and earthquake).The breeding colony will produce animals negative for Mycobacterium tuberculosis (TB), Simian Retrovirus(SRV), Simian T-Lymphotrophic Virus (STLV), Simian Immunodeficiency Virus (SIV), and Cercopithecine Herpes Virus 1 (CHV-1). This SPF status will allow a broader range of research use for progeny animals, and provides a significant benefit to research staff by removal of latent zoonotic transmission of CHV-1, a potentially lethal pathogen. In addition to their SPF status, the monkeys will be fed a custom-prepared diet free of pesticides and other endocrine disrupters. Most commercial monkey chows contain isoflavones; these bind to estrogen receptors and have numerous biological actions. As part of the colony research program to understand better how diet many influence fetal programming and later chronic disease risk, half of the monkeys will be fed an isoflavone-free diet and half fed a diet with isoflavones at levels similar to those found in commercial chow. These conditions will provide a unique resource of both SPF and diet-defined Macaca fascicularis available for NIH-sponsored biomedical research.

描述（由申请人提供）：人们普遍认为非人类灵长类动物是生物医学研究的宝贵资源。食蟹猴（Macaca fascicularis）是两种应用最广泛的猕猴物种之一，在传染病和免疫紊乱、糖尿病和心血管疾病、妇女健康、骨质疏松症和生殖道癌症等领域具有生物医学应用。我们提议扩大现有的无特定病原体 (SPF) 食蟹猴示范群落，使其成为 NIH 受资助者的国家资源。该提案涉及维克森林大学医学院 (WFUSM) 和华盛顿国家灵长类动物研究中心 (WaNPRC) 之间的合作。 WFUSM 将提供基础设施、群体管理、遗传监测和慢性疾病模型（心血管、骨骼、代谢和肿瘤疾病）方面的专业知识，而 WaNPRC 则提供病毒学、免疫学和 SPF 育种方面的专业知识。建立具有明确病原体状态的国内繁殖群将提供许多优势，包括直接获得来源动物以及避免因政治动荡、恐怖主义、运输延误和国际检疫而可能造成的供应中断。该群体将位于自然灾害（洪水、飓风、龙卷风和地震）风险较低的地区。繁殖群体将生产出结核分枝杆菌（TB）、猿猴逆转录病毒（SRV）、猿猴T淋巴细胞病毒阴性的动物(STLV)、猿猴免疫缺陷病毒 (SIV) 和猴疱疹病毒 1 (CHV-1)。这种 SPF 状态将允许对子代动物进行更广泛的研究用途，并通过消除潜在的人畜共患 CHV-1（一种潜在的致命病原体）的潜在人畜共患传播，为研究人员提供显着的好处。除了 SPF 状态外，这些猴子还将接受不含农药和其他内分泌干扰物的定制饮食。大多数商业猴粮都含有异黄酮。它们与雌激素受体结合并具有多种生物学作用。作为群体研究计划的一部分，为了更好地了解饮食如何影响胎儿的编程和后来的慢性疾病风险，一半的猴子将被喂食不含异黄酮的饮食，另一半则喂食含有异黄酮的饮食，其含量与商业饲料中的水平相似。这些条件将为 NIH 资助的生物医学研究提供 SPF 和饮食定义的食蟹猴的独特资源。