MRI CORE

核磁共振核心

基本信息

批准号：
7960006
负责人：
Rheal A Towner
金额：
$ 14.36万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7960006
关键词：
Ablation Angiography Animals Bioenergetics Biomedical Research Brain Brain Neoplasms Breast Choline Computer Retrieval of Information on Scientific Projects Database Contrast Media Creatine Development Disease Experimental Animal Model Funding Grant Image Institution Investigation Lasers Lesion Lipids Liquid substance Magnetic Resonance Imaging Magnetic Resonance Spectroscopy Magnetism Malignant Neoplasms Malignant neoplasm of brain Metabolic Methods Microscopic Microscopy Modeling Molecular Molecular Target Monitor Mus Oklahoma Organ Pathogenesis Phase Phospholipids Rattus Research Research Personnel Resolution Resources Rodent Source Spectrum Analysis Techniques Technology Temperature Therapeutic Agents Tissues United States National Institutes of Health Vascular System base design imaging modality imaging probe in vivo instrument magnetic field melanoma tumor

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. At OMRF and OUHSC we utilize high-resolution MR techniques (including microscopic imaging and spectroscopy) for biomedical research. The use of rodent experimental animal models has dramatically advanced our ability to analyze and understand the molecular basis of various diseases, however few methods exist to be able to visualize the various tissues, organs and vascular systems of rodents in vivo and non-invasively at any meaningful resolution. The following instrument, a Bruker Biospin Biospec 70/30 USR horizontal magnetic small animal imaging spectrometer, is capable of obtaining in vivo microimages (at ~100 ¿m resolution or better for microscopic MRI) in rodent experimental animal models, and in addition provide in vivo functional information on molecular targets (contrast-enhanced MRI) as well as structural information on metabolites, such as lipids/phospholipids, bioenergetic compounds, creatine, choline, and lactate (using magnetic resonance spectroscopy (MRS)). Magnetic resonance imaging (MRI), and its related techniques in biomedical research, such as MR angiography (MRA), MR spectroscopy (MRS), have developed over the past few years with significant advances in high-field magnets, high-strength magnetic field gradients, imaging probe designs and tissue-specific contrast agents, which have allowed selective morphological, functional and metabolic investigations in mice and rats to be possible. Another recent advancement in MR technology has been the development of MR microscopy. Images can be obtained with in-plane resolutions better than 100 micron, and in-vivo spectra can be collected with sensitivity and spectral resolution previously obtained only by narrow-bore liquid NMR spectrometers. Morphological MRI and/or microscopic MRI (~100 ¿m resolution) is currently used in our facility to localize and determine the extent of brain cancer lesions in a rat model (Towner) and breast and/or melanoma cancer lesions in rats and mice (Chen), and to monitor temperature changes in laser-induced tumor ablation with the use of phase-sensitive and chemicla-shift imaging (CSI) methods(Chen). Magnetic resonance spectroscopy (MRS), using image-guided MRS, is also used to monitor metabolic profiles for rat brain tumor pathogenesis (lipid and brain metabolite alterations; Towner), as well as assess therapeutic agents (Towner).

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以出现在其他 CRISP 条目中列出的机构是。中心，不一定是研究者的机构。在 OMRF 和 OUHSC，我们利用高分辨率 MR 技术（包括显微成像和光谱学）进行生物医学研究。啮齿动物实验动物模型的使用极大地提高了我们分析和理解各种疾病分子基础的能力，但目前很少有方法可以分析和理解各种疾病的分子基础。能够以任何有意义的分辨率对啮齿动物体内的各种组织、器官和血管系统进行非侵入性可视化。以下仪器是 Bruker Biospin Biospec 70/30 USR 水平磁性小动物成像。光谱仪，能够在啮齿动物实验动物模型中获得体内显微图像（约 100 µm 分辨率或更好的显微 MRI），此外还提供分子靶标的体内功能信息（对比增强 MRI）以及结构信息代谢物，如脂质/磷脂、生物能化合物、肌酸、胆碱和乳酸（使用磁共振波谱 (MRS)）。磁共振成像（MRI）及其在生物医学研究中的相关技术，例如磁共振血管造影（MRA）、磁共振波谱学（MRS）在过去几年中得到了发展，在高场磁体、高强度磁场方面取得了重大进展梯度、成像探针设计和组织特异性造影剂使得对小鼠和大鼠进行选择性形态、功能和代谢研究成为可能。可以获得优于 100 微米的面内分辨率，并且可以以以前只能通过窄口径液体 NMR 光谱仪获得的灵敏度和光谱分辨率来收集体内光谱。目前在我们的设施中用于定位和确定大鼠模型 (Towner) 中脑癌病变的程度以及大鼠和小鼠 (Chen) 中乳腺癌和/或黑色素瘤癌病变的程度，以及使用相敏和化学位移成像 (CSI) 方法监测激光诱导肿瘤消融的温度变化 (Chen)。磁共振波谱 (MRS) 使用图像引导 MRS，也可用于监测代谢曲线。用于大鼠脑肿瘤发病机制（脂质和脑代谢物改变；Towner），以及评估治疗药物（Towner）。