Biochemical and Metabolic Profiling Core

生化和代谢分析核心

基本信息

批准号：
8001180
负责人：
LAWRENCE W CASTELLANI
金额：
$ 22.26万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8001180
关键词：
Address Adipocytes Adipose tissue Agar Gel Electrophoresis Antibody Affinity Apolipoproteins Biochemical Biological Assay Biological Markers Blood Pressure Body Composition Categories Cell Culture Techniques Cholesterol Cholesterol Esters DEXA Densitometry Embryo Fatty Acids Fibroblasts Gel Chromatography Glucose Glucose tolerance test Glycerol Glycogen Hepatocyte High Density Lipoprotein Cholesterol Human Resources In Vitro Insulin Ketone Bodies Leptin Life Lipids Lipoproteins Liver Measurement Metabolic Methodology Methods Mus Oxygen Consumption Pathway interactions Physiological Plasma Polyacrylamide Gel Electrophoresis Procedures Protein Biosynthesis Radiolabeled Sampling Soleus Muscle System Testing Thin Layer Chromatography Tissue Sample Tissues Triglycerides Ultracentrifugation Western Blotting adiponectin base density design experience fast protein liquid chromatography glucose metabolism in vivo intraperitoneal radiotracer respiratory

项目摘要

The Biochemical and Metabolic Profiling Core provides sensitive and accurate in vitro biochemical assays and in vivo metabolic/physiologic assays for the analysis of selected biomarkers relevant to lipid and glucose metabolism. The methods and assays described below have all been fully implemented and used successfully by our experienced personnel for several years. This Core has served as a vital component of the PPG for the past 20 years. The assays performed by the Biochemical and Metabolic Profiling Core can be broken down into four broad categories: 1). Analysis of plasma samples; 2) Analysis of tissue samples; 3) In vivo metabolic/physiologic tests. 4) Specialized biochemical and metabolic analysis designed to address specific project needs. The assays and methods we will use are listed below. The assays in categories 1-3 which will be used on a routine basis have a more detailed description of the methodology in the "Method Details" at the end of this section.

生化和代谢分析核心提供灵敏、准确的体外生化测定和体内代谢/生理测定，用于分析与脂质和葡萄糖代谢相关的选定生物标志物。下面描述的方法和测定已被我们经验丰富的人员充分实施和成功使用了几年。过去 20 年来，该核心一直是 PPG 的重要组成部分。生化和代谢分析核心执行的检测可分为四种大类：1）。血浆样本分析； 2）组织样本分析； 3) 体内代谢/生理测试。 4) 专门的生化和代谢分析，旨在满足特定的项目需求。下面列出了我们将使用的测定和方法。将在常规基础上使用的类别 1-3 中的测定在本节末尾的“方法详细信息”中有更详细的方法描述。