CORE--PHENOTYPIC MARKERS

核心——表型标记

• 批准号: 6564847
• 负责人: LAWRENCE W CASTELLANI
• 金额: $ 23.48万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564847
• 关键词: apolipoproteins; biomedical facility; blood chemistry; blood lipid; blood lipoprotein; blood lipoprotein metabolism

A major effort of Core A is to analyze human and mouse plasmas and serum for lipoproteins and their component lipids and apolipoproteins. The lipid analyses employ automated micro-method techniques to determine levels of total cholesterol, HDL* cholesterol, unesterified cholesterol, triacyglycerols, glycerol banks, and unesterified fatty acids. Human plasma LDL and VLDL concentrations including apoB, apoAI, apoAII, and Lp(a). In addition to the measurement of lipids, lipoproteins, and apolipoproteins, the Phenotypic Marker Core also determines levels of several other plasma factors related to lipid/lipoprotein metabolism and atherosclerosis, including plasma concentrations of insulin, glucose, leptin, and the enzymatic activities of lipoprotein lipase and hepatic lipase. All of the assays required to determine the phenotypes proposed in this Program Project are currently in use in our laboratory.

Core A 的主要工作是分析人和小鼠血浆和血清中的脂蛋白及其脂质成分和载脂蛋白。脂质分析采用自动微量方法技术来测定总胆固醇、HDL* 胆固醇、未酯化胆固醇、三酰甘油、甘油库和未酯化脂肪酸的水平。人血浆 LDL 和 VLDL 浓度，包括 apoB、apoAI、apoAII 和 Lp(a)。除了测量脂质、脂蛋白和载脂蛋白外，表型标记核心还可以测定与脂质/脂蛋白代谢和动脉粥样硬化相关的其他几种血浆因子的水平，包括胰岛素、葡萄糖、瘦素的血浆浓度和脂蛋白的酶活性脂肪酶和肝脂肪酶。我们的实验室目前正在使用确定本计划项目中提出的表型所需的所有测定方法。