CORE--LABORATORY ANALYSIS

核心——实验室分析

基本信息

批准号：
6189163
负责人：
LAWRENCE W CASTELLANI
金额：
$ 35.01万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-08-01 至 2000-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6189163
关键词：
animal tissue biomedical facility blood lipoprotein clinical chemistry human tissue

项目摘要

A major effort of the Laboratory Core is to analyze human and mouse plasma and sera for lipoproteins and their component lipids and apolipoproteins. The lipid analyses employ automated micromethod techniques that have been described previously to determine plasma levels of total cholesterol, HDL cholesterol, unesterified cholesterol, tricyglycerols, glycerol blanks, and unesterified fatty acids (1-5). Human plasma LDL and VLDL concentrations are calculated using Freidewald's formula (6). The Laboratory Core also routinely determines, human plasma apolipoprotein concentrations including apoB, apoAI, apoAII, Lp(a), and apoJ. In addition to the measurement of lipids, lipoproteins, and apolipoproteins, the Laboratory Core also determines levels of several other plasma factors related to lipid/lipoprotein metabolism and atherosclerosis, including the plasma concentrations of insulin, glucose, leptin and homocysteine, and the enzymatic activities of lipoprotein lipase, hepatic lipase, and plasma paraoxonase. All of the assays are components of the phenotyping extensively described in Projects 1 and 3 of this grant application and are currently in routine use in our laboratory. The accuracy of our lipid assays is certified by the CDC National Heart, Lung, and Blood Institute Lipid Standardization Program.

核心实验室的一项主要工作是分析人和小鼠血浆和血清中的脂蛋白及其脂质成分和载脂蛋白。脂质分析采用先前描述的自动化微量方法技术来测定总胆固醇、HDL 胆固醇、未酯化胆固醇、三环甘油、甘油空白和未酯化脂肪酸的血浆水平 (1-5)。人血浆 LDL 和 VLDL 浓度使用 Freidewald 公式 (6) 计算。实验室核心还定期测定人血浆载脂蛋白浓度，包括 apoB、apoAI、apoAII、Lp(a) 和 apoJ。除了测量脂质、脂蛋白和载脂蛋白外，实验室核心还测定与脂质/脂蛋白代谢和动脉粥样硬化相关的其他几种血浆因子的水平，包括胰岛素、葡萄糖、瘦素和同型半胱氨酸的血浆浓度以及酶活性脂蛋白脂肪酶、肝脂肪酶和血浆对氧磷酶。所有检测都是本资助申请项目 1 和 3 中广泛描述的表型分析的组成部分，目前在我们的实验室中常规使用。我们的脂质测定的准确性得到了 CDC 国家心脏、肺和血液研究所脂质标准化计划的认证。