A Resource Center for Tetrahymena Thermophila

嗜热四膜虫资源中心

• 批准号: 10591412
• 负责人: DOUGLAS LEE CHALKER
• 金额: $ 34.66万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10591412
• 关键词: Aging; Applied Research; Basic Science; Biological; Biological Phenomena; Biological Process; California; Cell Line; Cells; Cellular biology; Cloning Vectors; Clustered Regularly Interspaced Short Palindromic Repeats; Communities; Country; Data; Databases; Disease; Educational process of instructing; Engineering; Ensure; Eukaryotic Cell; Fertility; Functional disorder; Funding; Genetic; Genome; Genomics; Growth; Health; Human; Indiana; Infertility; Internet; Ion Channel; Laboratories; Maintenance; Malignant Neoplasms; Marketing; Mediating; Mentored Clinical Scientist Development Program; Modeling; Molecular Biology; Neurosecretory Systems; Nuclear; Online Systems; Organism; Play; Production; Recombinant Proteins; Research; Research Personnel; Resources; Retirement; Ribosomal DNA; Services; Study models; Support System; System; Tetrahymena; Tetrahymena thermophila; Universities; Vaccine Antigen; Washington; Water; cost; database schema; design; functional genomics; genetic information; genetic strain; genome analysis; genome database; genome editing; human disease; innovation; insight; interest; manufacture; migration; model organism; next generation; operation; practical application; preservation; repository; respiratory; therapeutic protein; tool; undergraduate student; user-friendly; vaccine platform; web site; wiki

Project Summary / Abstract This proposal seeks continued funding for the Tetrahymena Stock Center in order to maintain its current operations, expand its capabilities and ensure its sustainability as a resource for the community at-large. A key model for eukaryotic cell and molecular biology, Tetrahymena thermophila has been instrumental to our understanding of a wide range of biological phenomena with direct relevance to human health and disease including cancer, infertility, aging, respiratory and neuroendocrine dysfunction. Tetrahymena has also shown great promise as a platform for the production of recombinant proteins, including vaccine antigens and difficult- to-express human ion channels, and serves as a useful teaching tool in K12 and undergraduate classrooms. Specifically, our aims for the Resource are to 1) continue to act as a strain repository accepting new strains and making live cultures of T. thermophila available to interested users at reasonable cost; 2) complete the annotation of archival strains currently housed at the Center and acquire next-generation cloning vectors for micro- and macronuclear genome-editing; 3) offer expanded services and develop innovative marketing approaches to increase revenues. Additionally, to ensure insure long-term stability of the resource we plan to 4) relocate the repository from Cornell University to Washington University in St. Louis; 5) migrate all data from the legacy Tetrahymena Genome Database (TGD) schema to CHADO and transfer its web-hosting functions from Bradley University to the NSF-funded National Center for Genome Analysis Support (NCGAS) at Indiana University; and, 6) redesign the TGD website to display relevant data via the Tripal framework. Our additional Research aims are to: 7) develop genetic strains lacking a functional ribosomal DNA locus to enable selection-free creation of new cell lines; and, 8) develop CRISPR-mediated genome editing tools to enhance the overall utility of the Tetrahymena platform.

项目概要/摘要 该提案寻求继续为四膜虫库存中心提供资金，以维持其目前的水平 运营、扩大其能力并确保其作为整个社区的资源的可持续性。一把钥匙 真核细胞和分子生物学模型，嗜热四膜虫对我们的研究发挥了重要作用 了解与人类健康和疾病直接相关的各种生物现象 包括癌症、不孕症、衰老、呼吸和神经内分泌功能障碍。四膜虫还表现出 作为生产重组蛋白（包括疫苗抗原和困难蛋白）的平台，前景广阔 表达人类离子通道，并可作为 K12 和本科生课堂的有用教学工具。 具体来说，我们对该资源的目标是 1) 继续充当菌株存储库，接受新菌株和 以合理的成本向感兴趣的用户提供嗜热木霉的活培养物； 2）完成注释 目前保存在该中心的档案菌株，并获得用于微型和 大核基因组编辑； 3）提供扩展的服务并开发创新的营销方法 增加收入。此外，为了确保资源的长期稳定性，我们计划 4) 重新安置 从康奈尔大学到圣路易斯华盛顿大学的存储库； 5）迁移旧版的所有数据 四膜虫基因组数据库 (TGD) 架构到 CHADO 并从 Bradley 转移其网络托管功能 大学到 NSF 资助的印第安纳大学国家基因组分析支持中心 (NCGAS)；和， 6) 重新设计TGD网站，通过Tripal框架展示相关数据。我们的额外研究目标 目的是： 7) 开发缺乏功能性核糖体 DNA 位点的遗传菌株，以实现无选择的创造 新的细胞系； 8) 开发 CRISPR 介导的基因组编辑工具，以增强基因组编辑的整体效用 四膜虫平台。