Tissue Pathology and Biospecimen Shared Resource

组织病理学和生物样本共享资源

基本信息

批准号：
10627040
负责人：
Kar-Ming Fung
金额：
$ 13.4万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2028-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10627040
关键词：
Automation Basic Science Biological Markers Biological Specimen Banks Blood Cancer Biology Cancer Center Cancer Center Support Grant Cancer Control Catalogs Cells Certification Charge Clinical Trials Collaborations Collection Complex Computer software Computers Consultations Data Databases Dedications Detection Doctor of Philosophy Documentation Ensure Equipment Evaluation Experimental Designs Fees Fluorescence Formalin Fostering Freezing Fresh Tissue Funding Grant Histology Human Image Image Analysis Immunofluorescence Immunologic Immunohistochemistry In Situ Hybridization In Situ Nick-End Labeling Institution Intranet Laboratory Research Lasers Liquid substance Location Malignant Neoplasms Manuals Microscopy Neoplasm Circulating Cells Paraffin Paraffin Embedding Pathologist Pathology Patient Care Patient-Focused Outcomes Peer Review Population Heterogeneity Publications Quantitative Evaluations Reporting Reproducibility Research Research Personnel Research Project Grants Resource Sharing Retrieval S10 grant Sampling Scanning Secure Services Site Slide Specimen Stains Standardization Therapeutic Tissue Banks Tissue Embedding Tissue Microarray Tissue Sample Tissues Translating Translational Research United States National Institutes of Health Variant Work anticancer research arm biobank biomarker evaluation cancer prevention dashboard direct patient care experience histotechnology human tissue improved innovation interest member neoplastic cell operation patient privacy preservation prevent programs quality assurance recruit screening specific biomarkers success tissue processing waiver web site

项目摘要

TISSUE PATHOLOGY AND BIOSPECIMEN SHARED RESOURCE: ABSTRACT The Tissue Pathology and Biospecimen Shared Resource (TPB SR) provides histology services and procures, banks, and distributes human cancer and control tissue and fluid samples. The TPB SR is directed by Kar- Ming Fung, MD, PhD (CT), a pathologist who also serves as a liaison between SCC researchers and other pathologists. During the reporting period, the TPB SR completed a successful integration of the pre-existing SCC biospecimen repository into the TPB SR with a newly established annotated database and an online catalog. Other accomplishments include a new NIH/OD S10 grant and three local grants to establish fluorescence-based whole-slide scanning with image analysis and to expand the capabilities in automated in situ hybridization. To ensure quality and specialization, the TPB SR is organized into two arms. The tissue pathology arm provides: 1) consultation on experimental design and assistance in histopathologic interpretation; 2) a complete line of histology services from tissue processing, embedding, and sectioning paraffin and frozen bocks to construction of tissue / cell block microarrays; 3) automated single or multiplex immunohistochemistry, immunofluorescence, in situ hybridization and TUNEL using chromogen- or fluorescence-based detection; 4) bright-field and fluorescence whole-slide scanning with image analysis; and 5) isolation of circulating tumor cells and laser capture microscopy. The biospecimen arm provides: 1) procurement and banking services for fresh and fixed specimens with an annotated database; 2) an online collection catalog on the institutional intranet with a dash-board type display; and 3) search and retrieval of archival materials from the Department of Pathology. The website and data are stored in electronically and physically separated computers to prevent breach of information. Both arms are housed in SCC-designated space in the OUHSC’s laboratory research complex (BRC & BMSB), a convenient location for a majority (52 of 63 total) lab-based SCC members. From 2017 to 2021, usage by Cancer Biology (CB), Cancer Therapeutics (CT), and Cancer Prevention and Control (CPC) program members was 58%, 36% and 6%, respectively. During this period, SCC members (full and associate) accounted for 60% of total SR usage. SCC members with peer-reviewed support accounted for more than half (52%) of total SR usage, and 87% of SCC member usage. SCC affiliate members (i.e., non-CCSG members who conduct cancer-related work) accounted for another 20% of total usage. Data generated by the TPB SR was used to secure 11 peer-reviewed funded cancer research grants as well as many other grants and scientific publications. TPB SR services are offered free-of-charge to SCC members. This waiver of fees is approved on an annual basis by the SCC director and provides a significant value-added benefit for SCC members to foster research.

组织病理学和生物样本共享资源：摘要组织病理学和生物样本共享资源 (TPB SR) 提供组织学服务并采购、 TPB SR 由 Kar- 指导。 Ming Fung，医学博士、博士（CT），病理学家，同时也是 SCC 研究人员和其他人之间的联络人在报告期内，TPB SR 成功整合了现有的病理学家。 SCC 生物样本存储库进入 TPB SR，带有新建立的注释数据库和在线数据库其他成就包括一项新的 NIH/OD S10 拨款和三项地方拨款以建立基于荧光的全玻片扫描和图像分析，并扩展了自动化的功能为了确保原位杂交的质量和专业化，TPB SR 分为两个组织。病理学部门提供：1）实验设计咨询和组织病理学协助解释；2) 从组织处理、包埋到切片的完整组织学服务石蜡和冷冻盒构建组织/细胞块微阵列 3) 自动化单一或多重微阵列；免疫组织化学、免疫荧光、原位杂交和使用显色剂的 TUNEL 或基于荧光的检测；4) 明场和荧光全玻片扫描及图像分析； 5) 循环肿瘤细胞的分离和激光捕获显微镜生物样本臂提供： 1) 带有注释数据库的新鲜和固定样本的采购和银行服务；2) 在线；机构内部网上的馆藏目录，以仪表板式显示；以及 3) 搜索和检索；病理科的档案材料和数据以电子方式存储。物理上分开的计算机以防止信息泄露。两个臂都安装在 SCC 指定的计算机中。 OUHSC 实验室研究综合体（BRC 和 BMSB）的空间，对于大多数人（52 名 2017 年至 2021 年，总共 63 名实验室 SCC 成员，由癌症生物学 (CB)、癌症治疗学使用。 (CT) 和癌症预防与控制 (CPC) 计划成员分别为 58%、36% 和 6%。在此期间，SCC 会员（正式会员和准会员）占 SR 使用总量的 60%。具有同行评审支持的占 SR 使用总量的一半以上 (52%)，并且占 SCC 成员的 87% SCC 附属成员（即从事癌症相关工作的非 CCSG 成员）的使用情况。 TPB SR 生成的另外 20% 的数据用于获得 11 项经过同行评审的资助。 TPB SR 提供癌症研究资助以及许多其他资助和科学出版物服务。这项费用豁免每年由 SCC 董事批准并免费提供给 SCC 会员。为 SCC 成员促进研究提供了显着的增值效益。