Histology - lmmunohistochemistry Module

组织学-免疫组织化学模块

• 批准号: 8848389
• 负责人: Kar-Ming Fung
• 金额: $ 20.64万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8848389
• 关键词: Animal Model; Antibodies; Area; Biochemical; Cancer Biology; Cell model; Cultured Cells; Evaluation; Freezing; Funding; Histology; Life; Malignant Neoplasms; Mentors; Microscope; Microscopic; Microscopy; Modeling; Oklahoma; Paraffin Embedding; Phase; Research Personnel; Slide; Staining method; Stains; Tissues; Training; animal tissue; anticancer research; cellular imaging; instrument; tissue culture; tissue processing

The purpose of the Histology, Immunostaining, Microscopy Core is to provide to the Promising Junior Investigators (PJIs) and cancer funded investigators, tissue processing, embedding, sectioning, histochemical staining of mounted slides, developing immunohistochemical (IHC) (brighfield or fluorescent) staining for paraffin embedded and frozen tissues and immunocytochemical (ICC) (brighfield or fluorescent) staining for cultured cells, evaluation of new antibodies for IHC staining, biochemical and special staining. The core will provide technical support to all scientific investigators in the above said areas; with PJIs having first priority. The core will also provide training on and use of various microscopes including conventional bright field, phase and epifluorescence microscopy as well as new instruments for live cell imaging. This facility is critical for cancer investigators using animal models and tissue culture models to study basic mechanisms of cancer biology.

组织学、免疫染色、显微镜核心的目的是为有前途的初级研究人员 (PJI) 和癌症资助的研究人员提供组织处理、包埋、切片、固定载玻片的组织化学染色、开发免疫组织化学 (IHC)（明场或荧光）染色用于石蜡包埋和冷冻组织以及培养细胞的免疫细胞化学 (ICC)（明场或荧光）染色，评估用于 IHC 染色的新抗体，生化和特殊染色。核心将为上述领域的所有科研人员提供技术支持； PJI 具有第一优先权。该核心还将提供各种显微镜的培训和使用，包括传统的明场显微镜、相差显微镜和落射荧光显微镜以及用于活细胞成像的新仪器。该设施对于癌症研究人员使用动物模型和组织培养模型来研究癌症生物学的基本机制至关重要。