Dako Omnis Autostainer for Screening Tumor Tissues with Immunohistochemistry

Dako Omnis Autostainer 用于通过免疫组织化学筛查肿瘤组织

• 批准号: 8825649
• 负责人: Angela Omilian
• 金额: $ 17.5万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825649
• 关键词: Address; Antibodies; Automation; Basic Science; Biological Markers; Cancer Center Support Grant; Cell physiology; Detection; Ensure; Equipment; Funding; Histology; Housing; Humidity; Immunohistochemistry; In Situ Hybridization; Longevity; Malignant Neoplasms; Mediating; Mission; Neoplasm Metastasis; Pathology; Principal Investigator; Process; Reagent; Recording of previous events; Research; Research Personnel; Resource Sharing; Resources; Roswell Park Cancer Institute; Running; Slide; Staging; Staining method; Stains; Standardization; System; Temperature; Testing; Therapeutic Intervention; Time; Translational Research; Tumor Tissue; angiogenesis; base; cancer diagnosis; cost; cost effective; experience; human tissue; instrument; meetings; outcome forecast; prevent; protein expression; screening; tumor growth

 DESCRIPTION (provided by applicant): Roswell Park Cancer Institute (RPCI) seeks funds for the purchase of a Dako Omnis autostainer for immunohistochemistry (IHC). The instrument will be housed in the Pathology Resource Network facility (PRN) and overseen by Dr. Angela Omilian. The PRN is supported by a Cancer Center Support Grant and has been serving researchers as a formal shared resource core at RPCI for 7 years. The histology expertise associated with the PRN facility dates to even earlier; this facility has assisted investigators wih automated IHC since 2001. Thus, PRN staff is experienced and well-suited to operate and maintain the requested equipment. In a typical year, the PRN processes at least 7500 research IHC slides and uses two autostainers on a daily basis to meet this demand. Recently, one machine has been failing and is at the end of its useful lifespan. This is the impetus for our application. IHC is a straightforward, well-established, and cost-effective platform for the detection of protein expression in human tissue. In a research setting, IHC is often used to screen tumor tissues for biomarkers that can be used for cancer diagnosis, prognosis, staging, and/or as markers of therapeutic intervention. Automated IHC via the Dako Omnis permits an unprecedented level of standardization from run to run, quality, and also allows the PRN facility to process up to 120 research slides each workday, easily serving the needs of several investigators at RPCI at once. This application includes 16 principal investigators spanning a diverse range of basic and translational research, yet all sharing the common mission of RPCI: "To Understand, Prevent, and Cure Cancer." All users included in this application have a demonstrated history of using IHC to address translational research questions using biomarkers to signify the key cellular processes that are responsible for mediating tumor growth, angiogenesis, metastasis, and invasiveness. The requested Dako Omnis is superior to the current machinery used in the PRN for the following reasons: a) the Dako Omnis has temperature and humidity controls that permit an unprecedented level of standardization for antibody staining; b) the capability to run in situ hybridization simultaneously with IHC testing; ) an enhanced ability to run multiple slides for multiple investigators simultaneously and with ease; d) an increased slide capacity and shorter processing time allowing for greater throughput; e) uses less reagents permitting more slides per study for the same cost; f) a higher level of automation that reduces errors and ensures consistency. The Dako Omnis addresses all of the aforementioned factors in a way that is more controlled and comprehensive than any other staining system to date.

 描述（由申请人提供）：罗斯威尔帕克癌症研究所 (RPCI) 寻求资金购买用于免疫组织化学 (IHC) 的 Dako Omnis 自动染色仪。该仪器将安置在病理资源网络设施 (PRN) 中，并由 Angela 博士监督。 Omilian。PRN 得到癌症中心支持拨款的支持，7 年来一直为研究人员提供与 RPCI 相关的组织学专业知识。 PRN 设施的历史更早；自 2001 年以来，该设施一直在协助研究人员进行自动化 IHC。因此，PRN 工作人员经验丰富，非常适合操作和维护所需的设备。在典型的一年中，PRN 至少处理 7500 张研究 IHC 载玻片。每天使用两台自动染色机来满足这一需求，最近，一台机器出现故障并且已达到其使用寿命，这是我们应用的动力。用于检测人体组织中蛋白质表达的简单、完善且经济有效的平台在研究环境中，IHC 通常用于筛选肿瘤组织中的生物标志物，这些生物标志物可用于癌症诊断、预后、分期和/或。或作为治疗干预的标记，通过 Dako Omnis 实现了运行之间前所未有的标准化水平，还允许 PRN 设施每个工作日处理多达 120 张研究载玻片，轻松满足多个研究人员的需求。该应用程序包括 16 位主要研究人员，涵盖各种基础研究和转化研究，但所有人都承担着 RPCI 的共同使命：“了解、预防和治愈癌症”。使用 IHC 来解决转化研究问题，使用生物标志物来表示负责介导肿瘤生长、血管生成、转移和侵袭性的关键细胞过程。所要求的 Dako Omnis 优于目前使用的机器。 PRN 的原因如下：a) Dako Omnis 具有温度和湿度控制功能，可实现前所未有的抗体染色标准化；b) 能够与 IHC 测试同时运行原位杂交；增强了运行多个载玻片的能力；多个研究人员同时轻松进行； d) 增加载玻片容量并缩短处理时间，从而提高通量； e) 使用更少的试剂，以相同的成本允许每次研究使用更多的载玻片； f) 更高水平的自动化，减少错误并确保一致性。这Dako Omnis 以比迄今为止任何其他染色系统更可控、更全面的方式解决了所有提到的因素。