A modular platform for infectious disease surveillance at point-of-need.

用于按需监测传染病的模块化平台。

基本信息

批准号：
10633681
负责人：
Jay Kenneth Fisher
金额：
$ 99.9万
依托单位：
REDBUD LABS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-07 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10633681
关键词：
Africa Animal Model Automation Biological Assay Blood Blood specimen Boston Buffers Clinical Communicable Diseases Confusion Consensus Sequence Cytolysis Dangerousness Decision Making Dengue Dengue Fever Dengue Virus Deposition Detection Detergents Development Devices Diagnosis Diagnostic Differential Diagnosis Disease Disease Outbreaks Disease Surveillance Ebola Ebola virus Epidemiologic Monitoring Failure Family Filoviridae Infections Filovirus Flavivirus Freeze Drying Goals Health Resources Health system Infection Infrastructure Laboratories Malaria Marburgvirus Measures Methods Microfluidics Molecular Molecular Diagnostic Testing Nosocomial Infections Nucleic Acids Pan Genus Parasites Patient Triage Patients Performance Phase Plasmodium Plasmodium falciparum Public Health Quarantine RNA Reagent Research Response Elements Risk Running Sampling Sensitivity and Specificity Specificity Spottings Symptoms Syndrome System Systems Integration Test Result Testing Time Triage Universities Update Validation Viral Hemorrhagic Fevers Virus Whole Blood Yellow Fever Yellow fever virus assay development base biosafety level 4 facility care providers cost cross reactivity design falls genomic biomarker high risk improved infection risk innovation isothermal amplification microfluidic technology mortality patient population pre-clinical programs response success tissue culture viron

项目摘要

ABSTRACT We propose to develop a point-of-need system for differential molecular identification of filoviruses from syndromically similar infections. Our goal is to enable rapid, sensitive, and specific identification of quarantinable infections to reduce nosocomial risk and improve outbreak response. The platform will simultaneously test 12 genomic markers at the genus and strain level using a modular microfluidic design that allows for rapid panel update and expansion. In Phase I we will develop an assay for pan-filovirus (pFi), followed by expansion of the panel and system integration in Phase II. The expanded panel will add pan-flavivirus (pFa) and pan-Plasmodium (pPa) detection as well as strain-specific targets for Ebola, Marburg, Dengue, Yellow Fever, and Malaria. Strain specific tests will enable better patient triage/treatment during an outbreak and improve disease surveillance in non-outbreak settings. The panel will require a 50 μL sample of whole blood and will achieve highly specific detection in < 30 minutes. To achieve this goal, we will combine Redbud Labs’ expertise in microfluidics and systems with the diagnostic development expertise of the Diagnostics Program at PATH (Seattle, WA), and the VHF testing/processing capabilities of the Connor Lab at Boston University (part of the BSL-4 facilities at NEIDL).

抽象的我们建议开发一种需求点系统，用于对丝状病毒进行差异分子鉴定我们的目标是能够快速、灵敏和特异性地识别可隔离的感染。该平台将同时测试 12 种感染，以降低院内风险并改善疫情应对。使用模块化微流体设计在属和品系水平上进行基因组标记，可进行快速面板检测在第一阶段，我们将开发一种泛丝状病毒（pFi）检测方法，然后进行扩展。第二阶段扩展的面板和系统集成将添加泛黄病毒（pFa）和泛疟原虫。 (pPa) 检测以及埃博拉、马尔堡、登革热、黄热病和疟疾的菌株特异性目标。特定测试将有助于在疫情暴发期间更好地对患者进行分类/治疗，并改善疾病监测非爆发设置。该小组需要 50 µL 全血样本，并将在 30 分钟内实现高度特异性的检测。为了实现这一目标，我们将把紫荆花实验室在微流体和系统方面的专业知识与诊断技术相结合。 PATH（华盛顿州西雅图）诊断项目的开发专业知识以及 VHF 测试/处理波士顿大学康纳实验室（NEIDL BSL-4 设施的一部分）的能力。