A modular platform for infectious disease surveillance at point-of-need.

用于按需监测传染病的模块化平台。

• 批准号: 10697143
• 负责人: Jay Kenneth Fisher
• 金额: $ 98.36万
• 依托单位: REDBUD LABS, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-07 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10697143
• 关键词: Acceleration; Africa; Animal Model; Automation; Biological Assay; Blood; Blood specimen; Boston; Buffers; Categories; Clinical; Communicable Diseases; Confusion; Consensus Sequence; Cytolysis; Dangerousness; Decision Making; Dengue; Dengue Fever; Dengue Virus; Deposition; Detection; Detergents; Development; Devices; Diagnosis; Diagnostic; Differential Diagnosis; Disease; Disease Outbreaks; Disease Surveillance; Ebola; Ebola virus; Epidemiologic Monitoring; Failure; Family; Filoviridae Infections; Filovirus; Flavivirus; Freeze Drying; Goals; Health Resources; Health system; Incubated; Infection; Infrastructure; Laboratories; Malaria; Marburgvirus; Measures; Methods; Microfluidics; Molecular; Molecular Diagnostic Testing; Nosocomial Infections; Nucleic Acids; Pan Genus; Parasites; Patient Admission; Patient Triage; Patients; Performance; Phase; Plasmodium; Plasmodium falciparum; Public Health; Quarantine; RNA; Reagent; Research; Response Elements; Risk; Running; Sampling; Sensitivity and Specificity; Specificity; Spottings; Suspensions; Symptoms; System; Systems Integration; Testing; Time; Triage; Universities; Update; Validation; Viral Hemorrhagic Fevers; Virus; Whole Blood; Yellow Fever; Yellow fever virus; assay development; biosafety level 4 facility; care providers; cost; cross reactivity; design; falls; genomic biomarker; high risk; improved; infection risk; innovation; isothermal amplification; microfluidic technology; mortality; outbreak response; patient population; pre-clinical; programs; success; tissue culture; viron

ABSTRACT We propose to develop a point-of-need system for differential molecular identification of filoviruses from syndromically similar infections. Our goal is to enable rapid, sensitive, and specific identification of quarantinable infections to reduce nosocomial risk and improve outbreak response. The platform will simultaneously test 12 genomic markers at the genus and strain level using a modular microfluidic design that allows for rapid panel update and expansion. In Phase I we will develop an assay for pan-filovirus (pFi), followed by expansion of the panel and system integration in Phase II. The expanded panel will add pan-flavivirus (pFa) and pan-Plasmodium (pPa) detection as well as strain-specific targets for Ebola, Marburg, Dengue, Yellow Fever, and Malaria. Strain specific tests will enable better patient triage/treatment during an outbreak and improve disease surveillance in non-outbreak settings. The panel will require a 50 μL sample of whole blood and will achieve highly specific detection in < 30 minutes. To achieve this goal, we will combine Redbud Labs’ expertise in microfluidics and systems with the diagnostic development expertise of the Diagnostics Program at PATH (Seattle, WA), and the VHF testing/processing capabilities of the Connor Lab at Boston University (part of the BSL-4 facilities at NEIDL).

抽象的 我们建议开发一种需求点系统，用于对丝状病毒进行差异分子鉴定 我们的目标是能够快速、灵敏和特异性地识别可隔离的感染。 该平台将同时测试 12 种感染，以降低院内风险并改善疫情应对。 使用模块化微流体设计在属和品系水平上进行基因组标记，可进行快速面板检测 在第一阶段，我们将开发一种泛丝状病毒（pFi）检测方法，然后进行扩展。 第二阶段扩展的面板和系统集成将添加泛黄病毒（pFa）和泛疟原虫。 (pPa) 检测以及埃博拉、马尔堡、登革热、黄热病和疟疾的菌株特异性目标。 特定测试将有助于在疫情暴发期间更好地对患者进行分类/治疗，并改善疾病监测 非爆发设置。 该小组需要 50 µL 全血样本，并将在 30 分钟内实现高度特异性的检测。 为了实现这一目标，我们将把紫荆花实验室在微流体和系统方面的专业知识与诊断技术相结合。 PATH（华盛顿州西雅图）诊断项目的开发专业知识以及 VHF 测试/处理 波士顿大学康纳实验室（NEIDL BSL-4 设施的一部分）的能力。

项目成果

A modular microfluidic device that uses magnetically actuatable microposts for enhanced magnetic bead-based workflows.

一种模块化微流体装置，使用可磁驱动的微柱来增强基于磁珠的工作流程。

• 发表时间: 2023-01-17
• 影响因子: 6.1
• 作者: Kanies, Olivia S;Kremer, Katelyn R;Mason, Brittany M;Dudley, Mitchell G;Hlavay, Joseph M;Miller, Cass T;Spero, Richard Chasen;Fisher, Jay K
• 通讯作者: Fisher, Jay K