Imaging adult-born neurons in action using head-mounted minimicroscopes

使用头戴式微型显微镜对活动中的成年神经元进行成像

• 批准号: 9203497
• 负责人: MICHAEL R DREW
• 金额: $ 12.83万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-20 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9203497
• 关键词: Accelerometer; Address; Adult; Animal Model; Animals; Award; BRAIN initiative; Brain; Brain region; Calcium; Cells; Code; Cognition; Data; Development; Disease; Electrophysiology (science); Emotional disorder; Emotions; Engineering; Etiology; Figs - dietary; Fright; Functional Imaging; Generations; Head; Hippocampus (Brain); Human; Image; Imaging Techniques; Learning; Link; Mediating; Memory; Methods; Modeling; Monitor; Moods; Mus; Neurons; Newborn Infant; Patients; Pattern; Play; Population; Procedures; Process; Research; Research Training; Role; Site; Stream; System; Techniques; Technology; Testing; Time; Training; Transgenic Organisms; abstracting; adult neurogenesis; base; conditioned fear; dentate gyrus; emotion regulation; equipment training; granule cell; improved; in vivo; information processing; insight; instrument; neural patterning; neurogenesis; optogenetics; programs; public health relevance; relating to nervous system; research study; young adult

Project Summary/Abstract The hippocampus is one of a select few brain regions that retain the ability to generate neurons in adulthood. Research in human patients and animal models suggests that increases and decreases in neurogenesis alter memory function and contribute to the etiology and treatment of emotional disorders. Despite almost 15 years of research linking adult neurogenesis to memory and emotional regulation, almost nothing is known about the circuit and coding mechanisms through which adult neurogenesis influences these processes. The recent development (with BRAIN Initiative support) of head-mounted minimicroscopes provides an unprecedented opportunity to image activity of adult-born neurons as animals learn and behave. Under this award, we will acquire equipment and training that will enable our lab to sustainably incorporate miniscope imaging into our research program on adult neurogenesis. In addition, we will perform calcium imaging experiments in freely moving mice as they explore contexts and undergo contextual fear conditioning, a model learning paradigm that is sensitive to perturbations of adult neurogenesis. Using optogenetic silencing of adult-born neurons, we will characterize how adult-born neurons influence coding of context memory in dentate gyrus, a region required for context memory. These experiments will provide new and previously unattainable insights into the mechanisms through which adult-born neurons regulate hippocampal memory, and they will enable our lab to integrate miniscope technology in a sustainable way in multiple ongoing research streams.

项目摘要/摘要 海马是一个精选的大脑区域之一，它保留了成年后产生神经元的能力。 人类患者和动物模型的研究表明，神经发生的增加和减少 记忆功能并有助于情绪障碍的病因和治疗。尽管将近15年 关于将成人神经发生与记忆和情绪调节联系起来的研究，几乎一无所知 成人神经发生影响这些过程的电路和编码机制。最近 头部安装最小镜的开发（带有大脑倡议支持）提供了前所未有的 随着动物的学习和行为，有机会图像成年神经元的活动。根据该奖项，我们将 获取设备和培训，使我们的实验室能够可持续地将Miniscope成像纳入我们的 成人神经发生研究计划。此外，我们将在自由地执行钙成像实验 在探索环境并经历上下文恐惧条件时移动老鼠，模型学习范式 这对成人神经发生的扰动很敏感。使用成人出生神经元的光遗传沉默，我们 将表征成人出生的神经元如何影响牙齿回旋中上下文记忆的编码，一个区域 需要上下文内存。这些实验将为您提供新的和以前无法实现的见解 成年神经元调节海马记忆的机制，它们将使我们的实验室能够 以多种正在进行的研究流以可持续的方式整合Miniscope技术。